Detailed information of TA system TA074593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1075966..1076624
• Replicon: chromosome
• Accession: NZ_CP020395
• Organism: Burkholderia vietnamiensis strain FDAARGOS_239

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8H33_RS21195
• Protein ID: WP_043292779.1
• Coordinates: 1076193..1076624 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1B4LJE8
• Locus tag: A8H33_RS21190
• Protein ID: WP_043292778.1
• Coordinates: 1075966..1076193 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H33_RS21165	1071801..1072509	-	709	Protein_953	cupin domain-containing protein	-
A8H33_RS21170	1072695..1073102	-	408	WP_011880193.1	DUF934 domain-containing protein	-
A8H33_RS21175	1073099..1074889	-	1791	WP_011880194.1	nitrite/sulfite reductase	-
A8H33_RS21180	1075023..1075169	-	147	Protein_956	transposase	-
A8H33_RS21190	1075966..1076193	+	228	WP_043292778.1	hypothetical protein	Antitoxin
A8H33_RS21195	1076193..1076624	+	432	WP_043292779.1	PIN domain-containing protein	Toxin
A8H33_RS21200	1077039..1077470	+	432	WP_011880199.1	hypothetical protein	-
A8H33_RS21205	1077467..1078000	+	534	WP_011880200.1	RES family NAD+ phosphorylase	-
A8H33_RS21210	1078401..1079384	+	984	WP_080938772.1	tyrosine-type recombinase/integrase	-
A8H33_RS32320	1079381..1079764	+	384	WP_144410681.1	hypothetical protein	-
A8H33_RS21215	1079797..1080614	+	818	WP_085954470.1	IS5-like element IS402 family transposase	-
A8H33_RS21220	1080664..1081251	+	588	WP_080938773.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1072695..1157513	84818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15621.97 Da
• Isoelectric Point: 7.2342

>T74593 WP_043292779.1 NZ_CP020395:1076193-1076624 [Burkholderia vietnamiensis]
ITYLLDVNVLIALLDPAHVQHEPAHAWFGRVGHSAWATCPLTENGVLRIIGNPKYPNTMVSPASVAPLVVQLRSHAGHTF
WSDDVSLLDEKHVDATRLLSSAQLTDTYLLALARAHKGKLATFDRRLVVDAVPNGGKHLELIP

Nucleotide

• Length: 432 bp

>T74593 NZ_CP020395:1076193-1076624 [Burkholderia vietnamiensis]
ATTACCTACCTTCTCGACGTCAACGTACTCATTGCTTTGCTCGACCCGGCACACGTTCAACACGAGCCGGCGCATGCGTG
GTTTGGCCGGGTAGGCCATTCCGCATGGGCCACGTGCCCGCTCACCGAGAACGGCGTGCTTCGAATTATCGGCAATCCGA
AATACCCGAATACGATGGTCTCTCCTGCATCCGTTGCACCGCTTGTTGTGCAACTGCGGTCCCATGCTGGGCACACATTC
TGGTCGGACGATGTGAGCCTGCTCGACGAAAAACACGTCGACGCCACCCGTTTACTGTCCTCCGCTCAACTGACCGACAC
TTACCTCCTCGCGCTCGCTCGCGCACACAAAGGCAAGTTGGCGACCTTCGATCGCCGCTTGGTCGTCGACGCGGTTCCGA
ACGGCGGGAAGCATCTTGAACTGATTCCTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8080.14 Da
• Isoelectric Point: 8.9793

>AT74593 WP_043292778.1 NZ_CP020395:1075966-1076193 [Burkholderia vietnamiensis]
MRTTIDIDDDVLAFARERAKPGTSIGKVISDLARAALQRGTTNQSSRNGLPLMPVSATARPVTLDTVNQLRDDSL

Nucleotide

• Length: 228 bp

>AT74593 NZ_CP020395:1075966-1076193 [Burkholderia vietnamiensis]
ATGCGTACCACTATTGATATCGACGACGACGTCCTTGCCTTCGCGCGGGAGCGTGCAAAGCCCGGCACGAGTATCGGGAA
GGTCATTTCTGACCTCGCCCGTGCGGCCCTGCAGCGCGGAACAACGAACCAGTCGAGCCGAAACGGCTTGCCGTTGATGC
CGGTTTCCGCGACCGCCCGTCCGGTCACCCTTGATACCGTTAATCAACTTCGCGACGACTCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B4LJE8