Detailed information of TA system TA074589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 3041242..3041804
• Replicon: chromosome
• Accession: NZ_CP020394
• Organism: Burkholderia vietnamiensis strain FDAARGOS_239

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A8H33_RS14985
• Protein ID: WP_045579167.1
• Coordinates: 3041242..3041562 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1B4LIX1
• Locus tag: A8H33_RS14990
• Protein ID: WP_045579168.1
• Coordinates: 3041556..3041804 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H33_RS32140	3037052..3037336	-	285	WP_045579163.1	hypothetical protein	-
A8H33_RS14955	3037400..3037861	-	462	WP_126221127.1	hypothetical protein	-
A8H33_RS32145	3037909..3038355	-	447	WP_126221126.1	hypothetical protein	-
A8H33_RS32150	3038369..3038668	-	300	WP_126221125.1	hypothetical protein	-
A8H33_RS32155	3038953..3039150	-	198	WP_059889990.1	hypothetical protein	-
A8H33_RS32160	3039188..3039430	-	243	WP_126221124.1	hypothetical protein	-
A8H33_RS14970	3040401..3040643	-	243	WP_045579166.1	hypothetical protein	-
A8H33_RS14980	3041001..3041231	-	231	WP_080938562.1	hypothetical protein	-
A8H33_RS14985	3041242..3041562	-	321	WP_045579167.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A8H33_RS14990	3041556..3041804	-	249	WP_045579168.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A8H33_RS14995	3041930..3042601	-	672	WP_052691567.1	site-specific integrase	-
A8H33_RS15000	3042765..3043091	+	327	WP_045579170.1	H-NS histone family protein	-
A8H33_RS15005	3043222..3044013	+	792	WP_045579171.1	SOS response-associated peptidase family protein	-
A8H33_RS15010	3044256..3044582	-	327	WP_045579172.1	hypothetical protein	-
A8H33_RS15015	3044820..3045707	-	888	WP_045579173.1	ImmA/IrrE family metallo-endopeptidase	-
A8H33_RS15020	3045711..3046058	-	348	WP_012367381.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

• SpoVT-AbrB domain (9-44)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11921.20 Da
• Isoelectric Point: 10.5568

>T74589 WP_045579167.1 NZ_CP020394:c3041562-3041242 [Burkholderia vietnamiensis]
MVMRGEVWLVALDPTLGSEIQKTRPCVVVSPPEMHDHLRTVIVAPMTSKGRPAPFRIPVTFKRKHGLILLDQIRAVDKVR
LVKKEGAIADKTLLDTLRTLQEVFAE

Nucleotide

• Length: 321 bp

>T74589 NZ_CP020394:c3041562-3041242 [Burkholderia vietnamiensis]
ATGGTGATGCGCGGGGAAGTCTGGTTGGTCGCACTCGATCCGACGCTTGGTAGCGAGATTCAAAAGACGCGCCCCTGTGT
GGTCGTTTCACCTCCCGAAATGCACGACCACTTGCGCACAGTCATCGTCGCGCCGATGACTTCCAAGGGGCGTCCAGCGC
CGTTTCGGATTCCCGTGACGTTCAAGCGCAAACATGGTTTGATCCTTCTGGATCAGATTCGCGCAGTGGACAAGGTGCGC
CTGGTTAAGAAAGAGGGTGCAATTGCCGATAAAACGTTGTTGGATACCCTGCGGACCTTGCAGGAAGTCTTTGCGGAATA
G

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8979.19 Da
• Isoelectric Point: 4.4929

>AT74589 WP_045579168.1 NZ_CP020394:c3041804-3041556 [Burkholderia vietnamiensis]
MKTTIRRMGNSQGVLIPKPVLAQLGLESEVEMEVENDAIVLRRPKHKAREGWAEASKALAESGDDALVMGEFSNADDAEL
AW

Nucleotide

• Length: 249 bp

>AT74589 NZ_CP020394:c3041804-3041556 [Burkholderia vietnamiensis]
ATGAAGACGACGATTCGCAGAATGGGTAACTCGCAGGGCGTGCTGATTCCGAAGCCCGTCCTTGCGCAACTCGGGCTGGA
GAGCGAAGTGGAAATGGAGGTCGAGAACGACGCGATTGTTCTGCGCCGCCCGAAGCACAAAGCCCGTGAGGGGTGGGCGG
AAGCAAGCAAGGCTCTGGCCGAGAGCGGCGACGATGCGCTGGTTATGGGCGAGTTTTCGAACGCCGATGACGCGGAGCTT
GCATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B4LIX1