Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4270512..4271102 Replicon chromosome
Accession NZ_CP020388
Organism Pluralibacter gergoviae strain FDAARGOS_186

Toxin (Protein)


Gene name pemK Uniprot ID A0A251ZGU9
Locus tag A8H26_RS20315 Protein ID WP_048252834.1
Coordinates 4270512..4270844 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J5P671
Locus tag A8H26_RS20320 Protein ID WP_048252967.1
Coordinates 4270845..4271102 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8H26_RS20290 4266141..4267031 + 891 WP_043083115.1 LysR family transcriptional regulator -
A8H26_RS20295 4267028..4267963 - 936 WP_045287913.1 L,D-transpeptidase -
A8H26_RS20300 4268056..4269006 - 951 WP_043083111.1 HTH-type transcriptional regulator Cbl -
A8H26_RS20305 4269109..4270026 - 918 WP_043083109.1 nitrogen assimilation transcriptional regulator NAC -
A8H26_RS20315 4270512..4270844 - 333 WP_048252834.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A8H26_RS20320 4270845..4271102 - 258 WP_048252967.1 antitoxin Antitoxin
A8H26_RS20325 4271850..4273253 + 1404 WP_048252966.1 cytosine permease -
A8H26_RS20330 4273267..4274880 + 1614 WP_048252833.1 GMC family oxidoreductase N-terminal domain-containing protein -
A8H26_RS20335 4274938..4275840 - 903 WP_048252832.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11879.77 Da        Isoelectric Point: 10.1839

>T74577 WP_048252834.1 NZ_CP020388:c4270844-4270512 [Pluralibacter gergoviae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTMGVIRCDQPRTI
DIAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T74577 NZ_CP020388:c4270844-4270512 [Pluralibacter gergoviae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CGGCAGGTTTCACTGTTTCACTGGAAGAAGCGGGGACCAAAACAATGGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATCGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCGGACGCCGTTGTTAATGAGGTACTTGCCAGGCTGGATGC
GATTCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9378.87 Da        Isoelectric Point: 4.5799

>AT74577 WP_048252967.1 NZ_CP020388:c4271102-4270845 [Pluralibacter gergoviae]
MYTTRLKKVGGSIMLAVPPAVLKTLALTTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT74577 NZ_CP020388:c4271102-4270845 [Pluralibacter gergoviae]
ATGTATACAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGCGCT
GACGACGGACAGCGAAGTCGGCATGACCATTAATAATGGTTGCCTGATAATCGAACCGCAGAAACGGCCTCATTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCATGCTGAAATGAGCGAAGAGGACCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A251ZGU9

Antitoxin

Source ID Structure
AlphaFold DB A0A0J5P671

References