Detailed information of TA system TA074562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1223932..1224485
• Replicon: chromosome
• Accession: NZ_CP020388
• Organism: Pluralibacter gergoviae strain FDAARGOS_186

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A379CUA0
• Locus tag: A8H26_RS05925
• Protein ID: WP_048254026.1
• Coordinates: 1224171..1224485 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: A8H26_RS05920
• Protein ID: WP_048254027.1
• Coordinates: 1223932..1224168 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H26_RS05895	1219337..1220968	+	1632	WP_043081630.1	Na/Pi cotransporter family protein	-
A8H26_RS05900	1221006..1221818	-	813	WP_048254029.1	shikimate 5-dehydrogenase	-
A8H26_RS05905	1222046..1222918	+	873	WP_043081632.1	23S rRNA pseudouridine(2604) synthase RluF	-
A8H26_RS05910	1223137..1223466	+	330	WP_048254047.1	helix-turn-helix domain-containing protein	-
A8H26_RS05915	1223467..1223739	-	273	WP_048254028.1	DUF3811 domain-containing protein	-
A8H26_RS05920	1223932..1224168	+	237	WP_048254027.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A8H26_RS05925	1224171..1224485	+	315	WP_048254026.1	CcdB family protein	Toxin
A8H26_RS05930	1224530..1225453	-	924	WP_048254025.1	ketopantoate/pantoate/pantothenate transporter PanS	-
A8H26_RS05935	1225560..1227143	-	1584	WP_048254024.1	RNA repair transcriptional activator RtcR	-
A8H26_RS05945	1227590..1228717	+	1128	WP_048254023.1	slipin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11520.48 Da
• Isoelectric Point: 6.7131

>T74562 WP_048254026.1 NZ_CP020388:1224171-1224485 [Pluralibacter gergoviae]
MQFTVYGNTGKSAVYPLLIDVTSDIIGQLNRHIAIPLLPLEKYPASQRPERLIPLIRLSDGKEYAVMTHEMASIPVSVLG
PVFCDASHYRPQIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T74562 NZ_CP020388:1224171-1224485 [Pluralibacter gergoviae]
ATGCAATTTACCGTATATGGAAATACCGGAAAAAGTGCTGTTTATCCTTTACTGATTGACGTGACGAGCGACATCATCGG
ACAGCTCAATCGACATATTGCGATTCCACTTTTACCTTTAGAAAAGTATCCCGCAAGCCAGCGTCCTGAACGCCTGATCC
CGTTAATCAGGCTGAGTGACGGCAAGGAATATGCCGTCATGACGCATGAGATGGCGAGTATCCCTGTTAGCGTGCTGGGG
CCAGTGTTTTGCGATGCTTCTCACTACCGTCCGCAAATTAAAGCGGCGATAGATTTCCTTCTCGACGGCATTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8703.68 Da
• Isoelectric Point: 5.6329

>AT74562 WP_048254027.1 NZ_CP020388:1223932-1224168 [Pluralibacter gergoviae]
MSIKQRTTQSVTMTIDRNLLARAREAGINLSAVLAAALSAEVSQYETKKWQVENSAAIEALNQFHDEHGGFSDDYRTF

Nucleotide

• Length: 237 bp

>AT74562 NZ_CP020388:1223932-1224168 [Pluralibacter gergoviae]
ATGTCAATAAAACAGCGAACAACGCAGAGCGTTACTATGACCATCGATCGTAATCTTCTTGCGCGTGCGCGTGAGGCCGG
TATTAATCTCAGTGCTGTATTGGCTGCAGCGCTCAGTGCCGAAGTGAGCCAGTACGAAACAAAAAAATGGCAGGTAGAAA
ACAGCGCGGCTATTGAGGCGCTCAATCAATTCCACGATGAGCATGGCGGCTTCAGCGATGACTACAGGACATTTTAG

Structures

Toxin

• AlphaFold DB: A0A379CUA0