Detailed information of TA system TA074561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 811688..812304
• Replicon: chromosome
• Accession: NZ_CP020388
• Organism: Pluralibacter gergoviae strain FDAARGOS_186

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8H26_RS03860
• Protein ID: WP_106912772.1
• Coordinates: 811688..812059 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1S2BMV8
• Locus tag: A8H26_RS03865
• Protein ID: WP_043081486.1
• Coordinates: 812062..812304 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H26_RS03845	808925..809827	+	903	WP_043081490.1	formate dehydrogenase subunit beta	-
A8H26_RS03850	809824..810459	+	636	WP_043081489.1	formate dehydrogenase cytochrome b556 subunit	-
A8H26_RS03855	810731..811660	+	930	WP_043081488.1	formate dehydrogenase accessory protein FdhE	-
A8H26_RS03860	811688..812059	-	372	WP_106912772.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8H26_RS03865	812062..812304	-	243	WP_043081486.1	ribbon-helix-helix protein, CopG family	Antitoxin
A8H26_RS03870	812441..812731	-	291	WP_043081485.1	helix-turn-helix domain-containing protein	-
A8H26_RS03875	812979..813920	-	942	WP_043081484.1	fatty acid biosynthesis protein FabY	-
A8H26_RS03880	813953..814390	-	438	WP_043081483.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A8H26_RS03885	814387..815250	-	864	WP_043081482.1	virulence factor BrkB family protein	-
A8H26_RS03890	815255..815845	-	591	WP_048254743.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (16-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13443.48 Da
• Isoelectric Point: 9.9871

>T74561 WP_106912772.1 NZ_CP020388:c812059-811688 [Pluralibacter gergoviae]
MDGPAVFDTNILIDLFNNRSEAANAIEQRGPHRAVSLVTWMEVMAGAKKYGQEARTAGVFSLFEVVDINKEIARRSVSLR
ATYGMKLPDAIILATAQARGSALITRNTKDFAGIPGVITPYRF

Nucleotide

• Length: 372 bp

>T74561 NZ_CP020388:c812059-811688 [Pluralibacter gergoviae]
ATGGATGGCCCGGCCGTTTTCGATACTAATATCCTGATCGATCTCTTCAACAACCGCAGCGAGGCCGCCAATGCGATTGA
GCAACGCGGCCCGCACCGGGCGGTAAGTCTGGTGACCTGGATGGAGGTCATGGCGGGGGCGAAAAAGTACGGACAGGAGG
CCAGAACCGCTGGCGTGTTCAGCCTGTTCGAAGTCGTTGATATCAATAAAGAGATTGCCAGGCGCAGCGTCTCGCTGAGG
GCTACCTACGGCATGAAGCTCCCCGACGCCATTATACTGGCCACTGCGCAGGCGCGCGGCAGCGCGCTCATCACTCGCAA
TACCAAAGACTTCGCCGGTATTCCCGGCGTTATCACACCCTACCGGTTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9336.59 Da
• Isoelectric Point: 4.4247

>AT74561 WP_043081486.1 NZ_CP020388:c812304-812062 [Pluralibacter gergoviae]
ISMILETDVARILLDVSDDVIRRLDGLKQLRDRPRAELLREAIEQYLDRQSPSVLRDALGLWSDGGEDGLVYERKLREEW

Nucleotide

• Length: 243 bp

>AT74561 NZ_CP020388:c812304-812062 [Pluralibacter gergoviae]
ATAAGTATGATTTTGGAGACGGACGTGGCAAGAATCCTTCTTGATGTGTCTGATGACGTCATCAGGCGGCTTGATGGTCT
GAAGCAACTGAGGGACAGGCCGCGCGCGGAGCTGCTGCGCGAAGCGATAGAGCAGTATCTCGACAGGCAAAGCCCGTCGG
TGCTCAGAGACGCGCTCGGGCTGTGGAGCGACGGCGGGGAAGATGGCTTGGTTTACGAGCGCAAACTGCGCGAGGAGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1S2BMV8