Detailed information of TA system TA074557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3434895..3435482
• Replicon: chromosome
• Accession: NZ_CP020384
• Organism: Rhodovulum sp. MB263

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: B5V46_RS15900
• Protein ID: WP_080617504.1
• Coordinates: 3434895..3435185 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: B5V46_RS15905
• Protein ID: WP_080617505.1
• Coordinates: 3435195..3435482 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5V46_RS15880	3431563..3432318	-	756	WP_196774271.1	DUF1073 domain-containing protein	-
B5V46_RS15885	3432323..3432556	-	234	WP_080617503.1	hypothetical protein	-
B5V46_RS15890	3432556..3433812	-	1257	WP_196774272.1	phage terminase large subunit	-
B5V46_RS15895	3433908..3434417	-	510	WP_060836213.1	terminase small subunit	-
B5V46_RS15900	3434895..3435185	+	291	WP_080617504.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B5V46_RS15905	3435195..3435482	+	288	WP_080617505.1	putative addiction module antidote protein	Antitoxin
B5V46_RS15910	3435629..3435901	+	273	WP_080617506.1	BrnT family toxin	-
B5V46_RS15915	3435882..3436103	+	222	WP_155774098.1	BrnA antitoxin family protein	-
B5V46_RS15920	3436116..3436754	-	639	WP_080617508.1	hypothetical protein	-
B5V46_RS15925	3436765..3437409	-	645	WP_080617509.1	hypothetical protein	-
B5V46_RS15930	3437420..3437893	-	474	WP_080617510.1	single-stranded DNA-binding protein	-
B5V46_RS15935	3437893..3438441	-	549	WP_080617511.1	hypothetical protein	-
B5V46_RS19945	3438461..3438622	-	162	WP_155774099.1	hypothetical protein	-
B5V46_RS15940	3438621..3438917	+	297	WP_155774100.1	hypothetical protein	-
B5V46_RS15945	3438914..3439270	+	357	WP_080617513.1	hypothetical protein	-
B5V46_RS15950	3439267..3439530	+	264	WP_080617514.1	DUF2312 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3421482..3443021	21539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-95)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10925.69 Da
• Isoelectric Point: 10.0301

>T74557 WP_080617504.1 NZ_CP020384:3434895-3435185 [Rhodovulum sp. MB263]
MSYEIHQSAEFSKWLRKLKDRTAQARIAIRIARLEAGLMGDAKFFDGIGELRITYGPGYRVYFVQQGETIIILLCGGDKG
SQRRDIEKAKTMAKEI

Nucleotide

• Length: 291 bp

>T74557 NZ_CP020384:3434895-3435185 [Rhodovulum sp. MB263]
ATGAGTTACGAAATTCACCAGTCCGCCGAGTTCTCGAAATGGCTTCGCAAGCTGAAAGACCGCACGGCGCAAGCCCGCAT
CGCGATCAGGATCGCCCGGCTTGAAGCTGGCCTCATGGGCGACGCCAAGTTCTTCGACGGCATTGGTGAACTTCGGATCA
CCTACGGCCCGGGATACCGCGTCTACTTCGTCCAGCAGGGCGAGACGATCATCATCCTGCTCTGCGGAGGCGACAAAGGC
TCCCAGCGCAGGGACATCGAAAAGGCCAAGACCATGGCCAAGGAAATTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10023.46 Da
• Isoelectric Point: 5.6939

>AT74557 WP_080617505.1 NZ_CP020384:3435195-3435482 [Rhodovulum sp. MB263]
MPIETTRFDAAEYLDTPEAQAMLLSDAFETGDAAYIKHAIGIVARARGIKDTAEAAGITREALHRALGEKGNPRLSTILG
VTKALGLTLKVEASA

Nucleotide

• Length: 288 bp

>AT74557 NZ_CP020384:3435195-3435482 [Rhodovulum sp. MB263]
ATGCCTATCGAAACCACGCGCTTCGACGCGGCCGAATATCTCGACACGCCGGAAGCGCAAGCGATGCTGCTCTCCGACGC
CTTCGAGACGGGTGACGCCGCCTACATCAAGCACGCCATCGGGATCGTCGCCAGGGCGCGCGGGATCAAGGACACGGCCG
AGGCCGCCGGGATCACCCGGGAGGCGCTGCACCGCGCCTTGGGTGAGAAGGGAAACCCGAGGCTGTCTACTATCCTCGGC
GTGACGAAGGCGCTTGGGCTGACCTTGAAGGTCGAGGCGTCGGCCTGA