Detailed information of TA system TA074553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2315763..2316741
• Replicon: chromosome
• Accession: NZ_CP020383
• Organism: Bacillus cereus strain FORC60

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: FORC60_RS12355
• Protein ID: WP_000624974.1
• Coordinates: 2315763..2316500 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: FORC60_RS12360
• Protein ID: WP_000237818.1
• Coordinates: 2316613..2316741 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC60_RS12330	2311405..2312187	+	783	WP_016512842.1	class I SAM-dependent methyltransferase	-
FORC60_RS12335	2312345..2314021	-	1677	WP_048558851.1	alpha-keto acid decarboxylase family protein	-
FORC60_RS12340	2314138..2314620	+	483	WP_000191887.1	MarR family winged helix-turn-helix transcriptional regulator	-
FORC60_RS12345	2314787..2315524	+	738	WP_048558852.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FORC60_RS12355	2315763..2316500	+	738	WP_000624974.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FORC60_RS12360	2316613..2316741	+	129	WP_000237818.1	hypothetical protein	Antitoxin
FORC60_RS12365	2316814..2316990	+	177	WP_000852629.1	stage II sporulation protein SB	-
FORC60_RS12370	2317010..2317399	-	390	WP_048558853.1	YxeA family protein	-
FORC60_RS12375	2317611..2319080	+	1470	WP_000287547.1	beta-Ala-His dipeptidase	-
FORC60_RS12380	2319326..2320135	+	810	WP_070179292.1	C39 family peptidase	-
FORC60_RS12385	2320161..2320763	+	603	WP_000517268.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28375.07 Da
• Isoelectric Point: 8.3170

>T74553 WP_000624974.1 NZ_CP020383:2315763-2316500 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTHWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFTDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIIIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T74553 NZ_CP020383:2315763-2316500 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCACTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTACCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAACCCATCAGTATTCGGTACGATGG
AATGGCATACAGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAATTAAATACAAACTTTCGGGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTATTATTGATGGAAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT74553 WP_000237818.1 NZ_CP020383:2316613-2316741 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT74553 NZ_CP020383:2316613-2316741 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACCGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5