Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3720017..3720691 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag B5817_RS18230 Protein ID WP_003417282.1
Coordinates 3720017..3720445 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag B5817_RS18235 Protein ID WP_003417286.1
Coordinates 3720449..3720691 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS18205 3715839..3716240 - 402 WP_003417264.1 cytidine deaminase -
B5817_RS18210 3716381..3716815 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
B5817_RS18215 3716812..3717246 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
B5817_RS18220 3717375..3719147 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
B5817_RS18225 3719147..3719938 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
B5817_RS18230 3720017..3720445 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
B5817_RS18235 3720449..3720691 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
B5817_RS18240 3720813..3721427 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
B5817_RS18245 3721424..3722089 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
B5817_RS18250 3722090..3722623 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
B5817_RS18255 3722620..3722979 - 360 WP_080520868.1 4a-hydroxytetrahydrobiopterin dehydratase -
B5817_RS18265 3724449..3725513 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T74548 WP_003417282.1 NZ_CP020381:c3720445-3720017 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T74548 NZ_CP020381:c3720445-3720017 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT74548 WP_003417286.1 NZ_CP020381:c3720691-3720449 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT74548 NZ_CP020381:c3720691-3720449 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References