Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3123381..3123909 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag B5817_RS15390 Protein ID WP_044108447.1
Coordinates 3123381..3123695 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag B5817_RS15395 Protein ID WP_003901465.1
Coordinates 3123679..3123909 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS15370 3118791..3120479 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
B5817_RS15375 3120483..3120809 - 327 WP_080520881.1 hypothetical protein -
B5817_RS15380 3120982..3121569 + 588 WP_003914429.1 DUF3558 family protein -
B5817_RS15385 3121588..3123237 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
B5817_RS15390 3123381..3123695 - 315 WP_044108447.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B5817_RS15395 3123679..3123909 - 231 WP_003901465.1 antitoxin MazE Antitoxin
B5817_RS15400 3123952..3124995 - 1044 WP_080520880.1 DUF2293 domain-containing protein -
B5817_RS21670 3125186..3125461 + 276 WP_003911993.1 DUF1778 domain-containing protein -
B5817_RS21675 3125637..3125891 - 255 WP_003917684.1 hypothetical protein -
B5817_RS15415 3126039..3126443 + 405 WP_009938577.1 hypothetical protein -
B5817_RS15420 3126440..3126631 + 192 WP_003414184.1 hypothetical protein -
B5817_RS15425 3126695..3127984 + 1290 Protein_2916 transposase family protein -
B5817_RS15430 3128218..3128475 + 258 WP_003899489.1 hypothetical protein -
B5817_RS15435 3128580..3128891 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11195.82 Da        Isoelectric Point: 10.7779

>T74542 WP_044108447.1 NZ_CP020381:c3123695-3123381 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELA

Download         Length: 315 bp

>T74542 NZ_CP020381:c3123695-3123381 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT74542 WP_003901465.1 NZ_CP020381:c3123909-3123679 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT74542 NZ_CP020381:c3123909-3123679 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References