Detailed information of TA system TA074540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3083342..3084021
• Replicon: chromosome
• Accession: NZ_CP020381
• Organism: Mycobacterium tuberculosis strain MTB1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: B5817_RS15165
• Protein ID: WP_003414059.1
• Coordinates: 3083342..3083758 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: B5817_RS15170
• Protein ID: WP_003414061.1
• Coordinates: 3083755..3084021 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5817_RS15145	3079394..3080296	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
B5817_RS15150	3080365..3081117	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
B5817_RS15155	3081361..3081636	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
B5817_RS15160	3081633..3083255	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
B5817_RS15165	3083342..3083758	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
B5817_RS15170	3083755..3084021	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B5817_RS15175	3084047..3084442	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
B5817_RS15180	3084439..3084708	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
B5817_RS15185	3084718..3085812	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
B5817_RS15190	3085809..3086228	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
B5817_RS15195	3086302..3086781	-	480	WP_003414073.1	dihydrofolate reductase	-
B5817_RS15200	3086852..3087652	-	801	WP_003911953.1	thymidylate synthase	-
B5817_RS15205	3087808..3088545	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T74540 WP_003414059.1 NZ_CP020381:c3083758-3083342 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T74540 NZ_CP020381:c3083758-3083342 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT74540 WP_003414061.1 NZ_CP020381:c3084021-3083755 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT74540 NZ_CP020381:c3084021-3083755 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64