Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2930974..2931688 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag B5817_RS14245 Protein ID WP_003413460.1
Coordinates 2931248..2931688 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag B5817_RS14240 Protein ID WP_003413456.1
Coordinates 2930974..2931261 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS14205 2926396..2926641 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
B5817_RS14210 2926638..2927042 + 405 WP_003899389.1 type II toxin-antitoxin system VapC family toxin -
B5817_RS14215 2927259..2927879 + 621 WP_003413441.1 DUF4178 domain-containing protein -
B5817_RS14220 2927890..2928384 + 495 WP_003413444.1 DUF2617 family protein -
B5817_RS14225 2928381..2928812 + 432 WP_003899390.1 DUF4247 domain-containing protein -
B5817_RS14230 2928837..2929295 + 459 WP_003413451.1 DUF350 domain-containing protein -
B5817_RS14235 2929292..2930863 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
B5817_RS14240 2930974..2931261 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
B5817_RS14245 2931248..2931688 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
B5817_RS14250 2931709..2932464 - 756 WP_080520801.1 YebC/PmpR family DNA-binding transcriptional regulator -
B5817_RS14255 2932597..2933193 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
B5817_RS14260 2933201..2934046 - 846 WP_003413466.1 acyl-CoA thioesterase II -
B5817_RS14265 2934075..2934974 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
B5817_RS14270 2935102..2935776 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T74538 WP_003413460.1 NZ_CP020381:2931248-2931688 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T74538 NZ_CP020381:2931248-2931688 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT74538 WP_003413456.1 NZ_CP020381:2930974-2931261 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT74538 NZ_CP020381:2930974-2931261 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References