Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2869511..2870142 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag B5817_RS13950 Protein ID WP_003413174.1
Coordinates 2869765..2870142 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag B5817_RS13945 Protein ID WP_003413167.1
Coordinates 2869511..2869768 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS13910 2865425..2865739 + 315 WP_009937839.1 hypothetical protein -
B5817_RS13915 2866035..2867246 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
B5817_RS13925 2867373..2868032 + 660 WP_003900846.1 LppA family lipoprotein -
B5817_RS13930 2868029..2868691 + 663 WP_003900848.1 hypothetical protein -
B5817_RS13935 2868688..2868966 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
B5817_RS13940 2869059..2869472 + 414 WP_003413164.1 PIN domain nuclease -
B5817_RS13945 2869511..2869768 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
B5817_RS13950 2869765..2870142 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
B5817_RS13955 2870158..2870532 - 375 WP_003413177.1 hypothetical protein -
B5817_RS13960 2870632..2871027 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
B5817_RS13965 2871024..2871269 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
B5817_RS13975 2871680..2872099 - 420 WP_003413190.1 A24 family peptidase -
B5817_RS13980 2872111..2872920 - 810 WP_003413193.1 shikimate dehydrogenase -
B5817_RS13985 2872917..2874170 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
B5817_RS13990 2874163..2874675 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T74536 WP_003413174.1 NZ_CP020381:2869765-2870142 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T74536 NZ_CP020381:2869765-2870142 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTGCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT74536 WP_003413167.1 NZ_CP020381:2869511-2869768 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT74536 NZ_CP020381:2869511-2869768 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References