Detailed information of TA system TA074510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1026263..1027236
• Replicon: chromosome
• Accession: NZ_CP020381
• Organism: Mycobacterium tuberculosis strain MTB1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: B5817_RS05005
• Protein ID: WP_003404756.1
• Coordinates: 1026736..1027236 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: O05910
• Locus tag: B5817_RS05000
• Protein ID: WP_003898647.1
• Coordinates: 1026263..1026739 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5817_RS04980	1022110..1023381	-	1272	WP_003404747.1	PPE family protein	-
B5817_RS04985	1023396..1023695	-	300	WP_003404748.1	PE family protein	-
B5817_RS04990	1024139..1025920	+	1782	WP_003404749.1	BCCT family transporter	-
B5817_RS04995	1025917..1026174	+	258	WP_003404750.1	hypothetical protein	-
B5817_RS05000	1026263..1026739	+	477	WP_003898647.1	DUF1778 domain-containing protein	Antitoxin
B5817_RS05005	1026736..1027236	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
B5817_RS05015	1027549..1028868	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
B5817_RS05020	1029156..1029737	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
B5817_RS05025	1029737..1031389	+	1653	WP_003404761.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1025917..1078090	52173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T74510 WP_003404756.1 NZ_CP020381:1026736-1027236 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T74510 NZ_CP020381:1026736-1027236 [Mycobacterium tuberculosis]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17387.98 Da
• Isoelectric Point: 11.2467

>AT74510 WP_003898647.1 NZ_CP020381:1026263-1026739 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT74510 NZ_CP020381:1026263-1026739 [Mycobacterium tuberculosis]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTAGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2

Antitoxin

• AlphaFold DB: A5U0V8