Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 699909..700555 Replicon chromosome
Accession NZ_CP020381
Organism Mycobacterium tuberculosis strain MTB1

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag B5817_RS03205 Protein ID WP_003403137.1
Coordinates 699909..700322 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag B5817_RS03210 Protein ID WP_003403139.1
Coordinates 700319..700555 (-) Length 79 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5817_RS03185 695992..697542 + 1551 WP_003403119.1 MCE family protein -
B5817_RS03190 697594..697986 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
B5817_RS03195 697983..698240 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
B5817_RS03200 698423..699658 - 1236 WP_003403128.1 ATP-binding protein -
B5817_RS03205 699909..700322 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
B5817_RS03210 700319..700555 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
B5817_RS03215 700659..701165 - 507 WP_003900973.1 two component system sensor kinase HK1 -
B5817_RS03220 701279..701809 - 531 WP_003917320.1 two component system sensor kinase HK2 -
B5817_RS03225 701793..702488 - 696 WP_003918099.1 two-component system response regulator TcrA -
B5817_RS03230 702611..702922 + 312 WP_003403164.1 hypothetical protein -
B5817_RS03235 702994..703944 + 951 WP_003900184.1 DUF1259 domain-containing protein -
B5817_RS03240 704185..704769 + 585 WP_080520832.1 IS607-like element IS1536 family transposase -
B5817_RS03245 704771..705481 + 711 Protein_629 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-126)

Antitoxin

(3-43)


Sequences


Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T74499 WP_003403137.1 NZ_CP020381:c700322-699909 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T74499 NZ_CP020381:c700322-699909 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT74499 WP_003403139.1 NZ_CP020381:c700555-700319 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT74499 NZ_CP020381:c700555-700319 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TQ88


Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References