Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 490376..491013 Replicon chromosome
Accession NZ_CP020375
Organism Bacillus velezensis strain JTYP2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAJT_RS02525 Protein ID WP_003156187.1
Coordinates 490663..491013 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAJT_RS02520 Protein ID WP_003156188.1
Coordinates 490376..490657 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAJT_RS02500 486741..487340 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
BAJT_RS02505 487433..487798 + 366 WP_007609580.1 holo-ACP synthase -
BAJT_RS02510 487963..488970 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
BAJT_RS02515 489087..490256 + 1170 WP_032873001.1 alanine racemase -
BAJT_RS02520 490376..490657 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAJT_RS02525 490663..491013 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAJT_RS02530 491131..491952 + 822 WP_003156182.1 STAS domain-containing protein -
BAJT_RS02535 491957..492322 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BAJT_RS02540 492325..492726 + 402 WP_003156178.1 anti-sigma regulatory factor -
BAJT_RS02545 492738..493745 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
BAJT_RS02550 493809..494138 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BAJT_RS02555 494135..494617 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BAJT_RS02560 494583..495371 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
BAJT_RS02565 495371..495973 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T74461 WP_003156187.1 NZ_CP020375:490663-491013 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T74461 NZ_CP020375:490663-491013 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT74461 WP_003156188.1 NZ_CP020375:490376-490657 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT74461 NZ_CP020375:490376-490657 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References