Detailed information of TA system TA074460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-chpS/PRK09907-MazE
• Location: 8268..8851
• Replicon: plasmid pSTH1
• Accession: NZ_CP020374
• Organism: Shewanella khirikhana strain TH2012

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: STH12_RS21120
• Protein ID: WP_126169620.1
• Coordinates: 8522..8851 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: STH12_RS21115
• Protein ID: WP_126169619.1
• Coordinates: 8268..8522 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STH12_RS21090	4394..5425	+	1032	WP_126169615.1	hypothetical protein	-
STH12_RS21095	5658..6023	+	366	WP_126169667.1	type II secretion system protein	-
STH12_RS21100	6032..6544	+	513	WP_126169616.1	hypothetical protein	-
STH12_RS21105	6541..7506	+	966	WP_126169617.1	hypothetical protein	-
STH12_RS21110	7518..8195	+	678	WP_126169618.1	hypothetical protein	-
STH12_RS21115	8268..8522	+	255	WP_126169619.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
STH12_RS21120	8522..8851	+	330	WP_126169620.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
STH12_RS21125	8953..9948	+	996	WP_126169621.1	trypsin-like serine protease	-
STH12_RS21130	10011..10679	+	669	WP_126169622.1	hypothetical protein	-
STH12_RS21135	10866..11231	-	366	WP_126169668.1	hypothetical protein	-
STH12_RS21140	11360..11851	+	492	WP_126169669.1	conjugative transfer signal peptidase TraF	-
STH12_RS21145	11876..12127	+	252	WP_126169623.1	hypothetical protein	-
STH12_RS21150	12099..13007	+	909	WP_126169624.1	hypothetical protein	-
STH12_RS21155	13052..13435	-	384	WP_126169625.1	DNA-binding protein	-
STH12_RS21160	13446..13745	-	300	WP_126169626.1	type II toxin-antitoxin system HigB family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50369	50369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

• SpoVT-AbrB domain (6-45)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11984.94 Da
• Isoelectric Point: 9.6566

>T74460 WP_126169620.1 NZ_CP020374:8522-8851 [Shewanella khirikhana]
MSYTPKRGDIVWVDFDPSAGHEQAHHRPALVLSPEPFNKQIQLALVAPITSRVRGHGFEVAVDTDKTKGVVLCQQVKTID
YAARGIKFIEKAPVAVLNEVLAKVRVLVS

Nucleotide

• Length: 330 bp

>T74460 NZ_CP020374:8522-8851 [Shewanella khirikhana]
ATGAGTTACACCCCAAAGCGAGGCGACATTGTTTGGGTCGATTTTGATCCATCAGCGGGGCACGAGCAGGCGCATCATCG
TCCCGCTCTGGTGCTGTCGCCCGAGCCGTTCAACAAGCAAATTCAACTTGCTCTAGTCGCTCCCATTACCTCCAGAGTAA
GGGGGCATGGTTTCGAGGTGGCTGTAGATACCGATAAGACCAAAGGTGTTGTTTTGTGTCAACAAGTCAAGACTATTGAC
TATGCGGCAAGGGGCATCAAGTTTATCGAAAAAGCCCCGGTTGCGGTGCTAAATGAAGTGCTTGCAAAAGTGCGAGTGTT
GGTCAGTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8921.16 Da
• Isoelectric Point: 4.3021

>AT74460 WP_126169619.1 NZ_CP020374:8268-8522 [Shewanella khirikhana]
MQTSIKKWGNSAGAIIPAAVLKKAGFGMGDQVEIEVVQGRIVMSPATPTFTLDELLNASPKGAFELDSEDTQWLSSSPVG
KEEI

Nucleotide

• Length: 255 bp

>AT74460 NZ_CP020374:8268-8522 [Shewanella khirikhana]
ATGCAAACCAGCATCAAAAAATGGGGTAACTCTGCCGGAGCAATTATTCCTGCTGCCGTGTTAAAGAAGGCCGGATTCGG
TATGGGGGATCAAGTTGAAATAGAGGTTGTCCAAGGGCGTATTGTGATGAGCCCTGCAACCCCTACCTTCACTCTTGATG
AATTGCTCAATGCCAGTCCGAAAGGGGCTTTTGAACTTGATTCAGAAGATACTCAATGGCTGAGTTCGTCACCTGTCGGC
AAGGAAGAAATCTGA