Detailed information of TA system TA074457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 271276..271910
• Replicon: plasmid pTs485
• Accession: NZ_CP020372
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: THSYN_RS32450
• Protein ID: WP_100923187.1
• Coordinates: 271530..271910 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: THSYN_RS32445
• Protein ID: WP_100923186.1
• Coordinates: 271276..271530 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS32420	266525..267112	-	588	Protein_243	AAA family ATPase	-
THSYN_RS32430	267387..268406	+	1020	WP_100923326.1	WYL domain-containing protein	-
THSYN_RS32435	268415..269881	+	1467	WP_100923184.1	cobyric acid synthase	-
THSYN_RS32440	269907..271028	+	1122	WP_100923185.1	Fic family protein	-
THSYN_RS32445	271276..271530	+	255	WP_100923186.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
THSYN_RS32450	271530..271910	+	381	WP_100923187.1	type II toxin-antitoxin system VapC family toxin	Toxin
THSYN_RS32455	272164..273117	+	954	WP_100923327.1	cobalamin biosynthesis protein CobD	-
THSYN_RS32460	273303..274229	-	927	WP_100923188.1	alpha/beta fold hydrolase	-
THSYN_RS34650	274940..275179	-	240	WP_157818097.1	hypothetical protein	-
THSYN_RS32470	275491..276276	+	786	Protein_252	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiB1/vipA / hsiC1/vipB / hcp1 / clpV1	1..484824	484824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-121)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13552.38 Da
• Isoelectric Point: 4.4345

>T74457 WP_100923187.1 NZ_CP020372:271530-271910 [Candidatus Thiodictyon syntrophicum]
MVIDTSAIIAILRGEPESDRLQQSIAAAPRRRLSTASLLECRIVAANLEIAAELEAWLAAAAVEIEPFTADQCRLAAQAF
LDFGKGRHPAGLNFGDCFAYALAKQTGEPLLYKGDDFGRTDIATDN

Nucleotide

• Length: 381 bp

>T74457 NZ_CP020372:271530-271910 [Candidatus Thiodictyon syntrophicum]
ATGGTTATCGATACCTCAGCGATCATCGCCATCCTGCGTGGTGAACCCGAATCGGACCGGTTGCAGCAGTCGATCGCAGC
AGCACCTCGTCGGCGACTGAGCACCGCAAGCCTTCTTGAATGCCGGATCGTGGCGGCCAATCTGGAGATCGCCGCGGAAC
TGGAGGCCTGGCTCGCCGCGGCGGCGGTCGAGATCGAGCCATTCACTGCCGACCAATGCCGGTTGGCGGCCCAAGCCTTT
CTGGATTTCGGCAAGGGTCGCCATCCGGCAGGTCTCAACTTCGGTGACTGCTTCGCCTATGCCCTGGCCAAACAAACCGG
CGAGCCCCTACTCTACAAGGGGGACGATTTTGGTCGAACCGATATCGCCACAGACAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9337.60 Da
• Isoelectric Point: 7.2454

>AT74457 WP_100923186.1 NZ_CP020372:271276-271530 [Candidatus Thiodictyon syntrophicum]
MSLHIVNPEADRLALEIAQLTNESVSAVVVRALREQVKQLRETASRGARLEQMREIAARSAHKCCADRRSHEAIIGYDEN
GLPS

Nucleotide

• Length: 255 bp

>AT74457 NZ_CP020372:271276-271530 [Candidatus Thiodictyon syntrophicum]
ATGTCACTTCATATCGTCAACCCGGAAGCGGATCGCCTCGCCCTCGAGATTGCCCAACTGACCAACGAGAGCGTCTCGGC
CGTAGTCGTTCGCGCACTGCGCGAACAAGTAAAGCAGCTGCGCGAGACAGCGAGTCGCGGCGCCAGACTAGAGCAGATGC
GCGAGATCGCAGCCCGCAGTGCGCACAAGTGCTGCGCCGACCGGCGCAGTCACGAGGCAATCATCGGCTACGACGAAAAC
GGCCTGCCAAGCTGA