Detailed information of TA system TA074456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 175863..176666
• Replicon: plasmid pTs485
• Accession: NZ_CP020372
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: THSYN_RS31990
• Protein ID: WP_100923114.1
• Coordinates: 175863..176363 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: THSYN_RS31995
• Protein ID: WP_100923115.1
• Coordinates: 176367..176666 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS34625	171633..171788	+	156	WP_157818087.1	Rpn family recombination-promoting nuclease/putative transposase	-
THSYN_RS31975	171819..172007	+	189	WP_157818088.1	Rpn family recombination-promoting nuclease/putative transposase	-
THSYN_RS31980	172194..172418	+	225	WP_100923318.1	DUF4351 domain-containing protein	-
THSYN_RS31985	172660..175503	+	2844	WP_100923113.1	dynamin family protein	-
THSYN_RS31990	175863..176363	-	501	WP_100923114.1	GNAT family N-acetyltransferase	Toxin
THSYN_RS31995	176367..176666	-	300	WP_100923115.1	DUF1778 domain-containing protein	Antitoxin
THSYN_RS32000	176720..177349	-	630	WP_100923116.1	hypothetical protein	-
THSYN_RS32005	177336..178733	-	1398	WP_100923117.1	aminodeoxychorismate synthase component I	-
THSYN_RS32010	178993..180054	-	1062	WP_100923118.1	hypothetical protein	-
THSYN_RS32015	180038..181039	-	1002	WP_100923119.1	ATP-grasp domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiB1/vipA / hsiC1/vipB / hcp1 / clpV1	1..484824	484824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-93)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18453.07 Da
• Isoelectric Point: 9.9038

>T74456 WP_100923114.1 NZ_CP020372:c176363-175863 [Candidatus Thiodictyon syntrophicum]
MTVKGIAKLHSAHNTDGFDCGNPELDHFLRRFALTNQRANLTQTYVLSKGNDVIAYYSLTVGQANHQDAPRRVTEGLGRY
PVPLMILARLAVTRSEQGHGWGGSLLKDALRRTAQAADIAGIRALFVHAKDEAARAFYEHFNFRPSPADPYHLFLLMKDL
ARLLGP

Nucleotide

• Length: 501 bp

>T74456 NZ_CP020372:c176363-175863 [Candidatus Thiodictyon syntrophicum]
ATGACCGTCAAGGGAATCGCCAAGCTTCATTCGGCCCACAACACGGACGGCTTTGATTGCGGCAACCCCGAACTCGATCA
TTTCCTCCGGCGCTTCGCCTTAACCAACCAACGCGCAAACCTTACCCAGACCTATGTACTGAGTAAAGGCAACGACGTGA
TCGCCTATTACAGCCTCACGGTCGGGCAGGCCAACCATCAGGACGCGCCGCGCCGCGTGACGGAAGGGCTGGGCCGCTAT
CCCGTGCCGCTCATGATACTGGCACGTCTGGCGGTAACCCGCAGCGAGCAGGGCCACGGATGGGGCGGGTCCCTGCTGAA
AGATGCCTTACGCCGCACCGCGCAAGCCGCTGACATCGCGGGTATCCGGGCACTGTTCGTTCATGCCAAGGACGAAGCGG
CGCGCGCGTTTTACGAGCATTTCAACTTCCGCCCCAGTCCGGCCGATCCTTACCATCTGTTCTTGCTGATGAAAGACCTT
GCTCGGTTGTTGGGACCCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10936.48 Da
• Isoelectric Point: 4.8896

>AT74456 WP_100923115.1 NZ_CP020372:c176666-176367 [Candidatus Thiodictyon syntrophicum]
MSRNPEPRSERIDIRATPSAKRALLDAANASRKTVSEFLLDSGLEKAAELMAERRLFLLDDAQWAAFLAALDAPPQPMPR
LERLFAEPSAVEKALSEST

Nucleotide

• Length: 300 bp

>AT74456 NZ_CP020372:c176666-176367 [Candidatus Thiodictyon syntrophicum]
ATGTCCCGGAATCCTGAACCCCGCAGCGAACGCATCGACATTCGCGCCACACCGAGCGCGAAACGCGCCTTGCTGGACGC
GGCCAACGCGTCACGCAAGACGGTATCTGAATTCCTGCTGGACAGCGGCCTGGAGAAGGCCGCCGAACTCATGGCCGAGC
GGCGTTTGTTCCTGCTGGATGACGCGCAATGGGCGGCTTTCCTCGCGGCCCTCGACGCGCCCCCGCAGCCTATGCCACGA
CTGGAGCGCCTGTTCGCGGAGCCCAGCGCCGTGGAGAAAGCACTGAGTGAAAGCACCTGA