Detailed information of TA system TA074454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 340615..341161
• Replicon: plasmid pTs417
• Accession: NZ_CP020371
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: THSYN_RS30840
• Protein ID: WP_100922893.1
• Coordinates: 340615..340950 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: THSYN_RS30845
• Protein ID: WP_100922894.1
• Coordinates: 340937..341161 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS30820	335765..336025	-	261	WP_100922889.1	Arc family DNA-binding protein	-
THSYN_RS30825	336200..337375	-	1176	WP_100922890.1	BREX-2 system adenine-specific DNA-methyltransferase PglX	-
THSYN_RS30830	337459..337746	-	288	WP_100922891.1	hypothetical protein	-
THSYN_RS30835	337879..340356	-	2478	WP_100922892.1	BREX-2 system adenine-specific DNA-methyltransferase PglX	-
THSYN_RS30840	340615..340950	-	336	WP_100922893.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THSYN_RS30845	340937..341161	-	225	WP_100922894.1	CopG family transcriptional regulator	Antitoxin
THSYN_RS30850	341246..345481	-	4236	WP_100922895.1	BREX system serine/threonine kinase PglW	-
THSYN_RS30855	345667..345912	+	246	WP_172965415.1	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..416864	416864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 11964.87 Da
• Isoelectric Point: 10.8303

>T74454 WP_100922893.1 NZ_CP020371:c340950-340615 [Candidatus Thiodictyon syntrophicum]
MKRGDILYADLSPSVGSEINKRRPVLIVSNDANNRAASTVTILPITSNMTRVYPFEVALSAAESGLPKDSKAQAQQIRTI
AKERIPGPAVGRLAADKMRAIDTAIRLHLAL

Nucleotide

• Length: 336 bp

>T74454 NZ_CP020371:c340950-340615 [Candidatus Thiodictyon syntrophicum]
ATGAAGCGTGGTGACATCCTCTATGCCGACCTCAGCCCGAGTGTGGGGTCCGAGATCAACAAACGCCGCCCGGTGCTGAT
CGTGAGCAACGATGCCAATAATCGGGCGGCGAGCACGGTCACGATCCTGCCCATCACTTCCAACATGACACGCGTCTATC
CCTTTGAGGTGGCGCTTTCCGCTGCCGAATCCGGACTCCCCAAGGATTCCAAGGCGCAGGCCCAGCAGATCCGTACCATT
GCCAAGGAGCGCATCCCAGGGCCGGCGGTCGGTCGACTCGCGGCGGACAAGATGCGCGCAATCGACACGGCCATTCGCCT
GCACCTCGCGCTATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8374.37 Da
• Isoelectric Point: 4.8255

>AT74454 WP_100922894.1 NZ_CP020371:c341161-340937 [Candidatus Thiodictyon syntrophicum]
MRAEKLSISLTPEAVTLIETYRQAHAMKSRSQVIEHALRRLREEDLEAAYREAAAELDPDWAPTTGDGLAHEAW

Nucleotide

• Length: 225 bp

>AT74454 NZ_CP020371:c341161-340937 [Candidatus Thiodictyon syntrophicum]
ATGCGGGCAGAAAAGCTGTCAATCTCGCTCACCCCGGAGGCGGTGACACTGATCGAGACCTATCGTCAGGCCCATGCGAT
GAAGAGCCGGTCCCAGGTCATCGAGCACGCGCTACGCCGGCTGCGGGAGGAAGACCTCGAGGCCGCCTACCGTGAGGCGG
CGGCCGAGCTCGACCCGGACTGGGCGCCGACCACCGGCGACGGGCTTGCCCATGAAGCGTGGTGA