Detailed information of TA system TA074453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 112122..112669
• Replicon: plasmid pTs417
• Accession: NZ_CP020371
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: THSYN_RS29880
• Protein ID: WP_172965404.1
• Coordinates: 112122..112448 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: THSYN_RS29885
• Protein ID: WP_100922750.1
• Coordinates: 112445..112669 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS29865	107230..107424	+	195	WP_100922747.1	hypothetical protein	-
THSYN_RS29870	107687..109768	+	2082	WP_100922748.1	phosphate acetyltransferase	-
THSYN_RS29875	109937..111991	+	2055	WP_100922749.1	tetratricopeptide repeat protein	-
THSYN_RS29880	112122..112448	-	327	WP_172965404.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THSYN_RS29885	112445..112669	-	225	WP_100922750.1	antitoxin MazE family protein	Antitoxin
THSYN_RS29890	113135..113822	-	688	Protein_126	DUF1016 domain-containing protein	-
THSYN_RS29895	114277..114498	+	222	WP_100922751.1	HU family DNA-binding protein	-
THSYN_RS29900	114608..116068	-	1461	WP_157818035.1	ImmA/IrrE family metallo-endopeptidase	-
THSYN_RS34510	116065..116469	-	405	WP_157818036.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..416864	416864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (8-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11709.66 Da
• Isoelectric Point: 6.9780

>T74453 WP_172965404.1 NZ_CP020371:c112448-112122 [Candidatus Thiodictyon syntrophicum]
MMRGDFVTIAMQGDFGKPRPALVIQADQFGEHATVTVLPVTSTLVAAPLFRITVQPSAENGLQQPSQVMVDKAMTVKRDK
VGQAFGRIDGDTMIEVERCLAVFFGIAK

Nucleotide

• Length: 327 bp

>T74453 NZ_CP020371:c112448-112122 [Candidatus Thiodictyon syntrophicum]
ATGATGCGGGGGGACTTTGTGACCATCGCCATGCAAGGCGATTTCGGCAAGCCAAGACCCGCGTTGGTGATTCAGGCGGA
TCAATTCGGCGAGCACGCCACCGTCACCGTACTGCCGGTAACCAGCACGCTGGTTGCCGCGCCGCTGTTTCGGATCACGG
TTCAGCCGAGCGCCGAGAACGGCTTGCAGCAGCCGTCACAAGTGATGGTCGATAAGGCGATGACCGTGAAGCGCGACAAG
GTCGGGCAAGCCTTCGGGCGCATCGATGGGGATACTATGATAGAGGTTGAGCGTTGTTTGGCCGTTTTCTTTGGCATTGC
AAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8345.51 Da
• Isoelectric Point: 8.0324

>AT74453 WP_100922750.1 NZ_CP020371:c112669-112445 [Candidatus Thiodictyon syntrophicum]
MATIPVNARVQKHRDALRRAGLRPVQIWVPDTRRPDFAQECRRQSLIAAQADLADADMQRFMDEAVADVAGWTA

Nucleotide

• Length: 225 bp

>AT74453 NZ_CP020371:c112669-112445 [Candidatus Thiodictyon syntrophicum]
TTGGCAACGATTCCCGTGAACGCCCGTGTTCAAAAGCATCGCGATGCCTTGCGTAGGGCAGGACTTCGCCCGGTGCAAAT
CTGGGTGCCTGATACGCGACGGCCGGACTTTGCGCAAGAATGCCGCCGCCAGTCGCTGATTGCCGCTCAGGCGGATCTGG
CCGACGCGGATATGCAGCGATTCATGGATGAAGCCGTGGCGGATGTGGCTGGCTGGACGGCATGA