Detailed information of TA system TA074449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 5796812..5797440
• Replicon: chromosome
• Accession: NZ_CP020370
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: THSYN_RS24730
• Protein ID: WP_100921489.1
• Coordinates: 5797087..5797440 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: THSYN_RS24725
• Protein ID: WP_100921488.1
• Coordinates: 5796812..5797102 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS24725	5796812..5797102	+	291	WP_100921488.1	DUF1778 domain-containing protein	Antitoxin
THSYN_RS24730	5797087..5797440	+	354	WP_100921489.1	GNAT family N-acetyltransferase	Toxin
THSYN_RS24735	5797517..5797579	-	63	Protein_4931	PIN domain nuclease	-
THSYN_RS24745	5797856..5798314	-	459	WP_157818004.1	ISAs1 family transposase	-
THSYN_RS24750	5798355..5799617	-	1263	Protein_4933	ISAs1 family transposase	-
THSYN_RS24755	5799670..5799879	-	210	WP_100921490.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
THSYN_RS24760	5800395..5802026	-	1632	WP_100921491.1	nif-specific transcriptional activator NifA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-90)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12121.08 Da
• Isoelectric Point: 5.6082

>T74449 WP_100921489.1 NZ_CP020370:5797087-5797440 [Candidatus Thiodictyon syntrophicum]
MGDAVTLGAPQPLAAIHDCTGFAMPEPIPVVVLARLAVHCSWQGNGLGRALMQDAGQRIVGAADSIGIRGVLVQALDDAA
KGFYERLGFDPSPLDPMALMITLGDLRASLAGRVRSA

Nucleotide

• Length: 354 bp

>T74449 NZ_CP020370:5797087-5797440 [Candidatus Thiodictyon syntrophicum]
ATGGGAGACGCCGTGACGCTCGGCGCGCCGCAACCGCTGGCCGCCATACATGACTGCACCGGGTTCGCTATGCCCGAGCC
GATCCCGGTGGTCGTGCTGGCCCGTCTGGCCGTCCACTGTTCCTGGCAGGGAAATGGGCTCGGCCGGGCCCTGATGCAGG
ATGCCGGACAGCGGATTGTCGGCGCCGCGGACAGCATCGGCATCCGTGGCGTGCTCGTGCAGGCCCTCGACGATGCCGCC
AAGGGGTTCTATGAGCGCCTTGGCTTCGACCCCTCACCGCTCGACCCGATGGCACTGATGATCACCCTGGGCGACCTTAG
GGCAAGTCTCGCCGGTCGCGTTCGGTCAGCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10765.29 Da
• Isoelectric Point: 7.9940

>AT74449 WP_100921488.1 NZ_CP020370:5796812-5797102 [Candidatus Thiodictyon syntrophicum]
MPTVQEPAAKRQTLNLRIKPEERELIDRAARASGKNRTEFILDATRRAAEDALLDQALLRVGAEAFAAFQARLDAPPQPN
ERLCKTMQSVPPWETP

Nucleotide

• Length: 291 bp

>AT74449 NZ_CP020370:5796812-5797102 [Candidatus Thiodictyon syntrophicum]
ATGCCGACAGTCCAAGAACCAGCCGCCAAGCGCCAGACGCTCAACCTGCGCATCAAACCCGAGGAACGCGAGCTGATCGA
TCGCGCCGCCCGTGCCAGCGGTAAGAACCGCACCGAGTTCATCCTCGATGCCACCCGCCGGGCCGCCGAGGACGCGCTGC
TCGACCAGGCCCTGCTGCGCGTCGGAGCCGAGGCCTTCGCCGCCTTCCAGGCACGGCTCGATGCCCCACCGCAACCCAAT
GAACGACTGTGCAAGACCATGCAGAGTGTGCCGCCATGGGAGACGCCGTGA