Detailed information of TA system TA074444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 4972454..4973134
• Replicon: chromosome
• Accession: NZ_CP020370
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: THSYN_RS21030
• Protein ID: WP_100920849.1
• Coordinates: 4972454..4972888 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: THSYN_RS21035
• Protein ID: WP_100920850.1
• Coordinates: 4972892..4973134 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS21015	4967895..4968779	-	885	WP_157817827.1	hypothetical protein	-
THSYN_RS21020	4968845..4970560	-	1716	WP_100918684.1	hypothetical protein	-
THSYN_RS21025	4970763..4972427	-	1665	WP_100920848.1	DEAD/DEAH box helicase	-
THSYN_RS21030	4972454..4972888	-	435	WP_100920849.1	type II toxin-antitoxin system VapC family toxin	Toxin
THSYN_RS21035	4972892..4973134	-	243	WP_100920850.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
THSYN_RS21040	4973573..4974166	+	594	WP_100920851.1	malonic semialdehyde reductase	-
THSYN_RS21045	4974324..4974530	+	207	WP_100922527.1	twin-arginine translocation signal domain-containing protein	-
THSYN_RS21055	4974937..4975923	+	987	WP_100920852.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4967895..4984650	16755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15640.00 Da
• Isoelectric Point: 4.8947

>T74444 WP_100920849.1 NZ_CP020370:c4972888-4972454 [Candidatus Thiodictyon syntrophicum]
MDGLRYLLDTNILSALIRQPQGPVAAMLARRGYGTVCTSIVVAAELCFGARKLGSSVLTRKVDELLASLPVLPLEAGVDE
TYAQSRLQLERAGTPIGPNDLPIAAHALDLGLTLVTDNVDEFTRVSRLRVENWLDDLTPVRDSR

Nucleotide

• Length: 435 bp

>T74444 NZ_CP020370:c4972888-4972454 [Candidatus Thiodictyon syntrophicum]
ATGGACGGGCTGCGCTACCTCCTCGACACCAACATCCTGTCCGCGCTGATCCGCCAACCCCAAGGGCCGGTCGCCGCGAT
GCTCGCCCGGCGTGGTTACGGCACCGTCTGCACCAGCATCGTCGTCGCCGCGGAACTGTGCTTCGGTGCCCGCAAGCTCG
GTTCCAGCGTGCTCACCCGGAAGGTCGATGAACTGCTGGCATCACTGCCCGTCCTGCCCCTGGAGGCCGGGGTCGATGAG
ACCTATGCCCAGTCCCGCCTTCAGTTGGAGCGAGCCGGGACGCCCATCGGACCCAACGACCTGCCGATTGCCGCACACGC
ACTGGACCTGGGGCTCACCCTCGTTACCGACAATGTGGACGAGTTCACGCGGGTTTCCAGATTGCGGGTGGAGAATTGGT
TAGACGATCTTACCCCGGTACGCGACAGCAGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9269.64 Da
• Isoelectric Point: 4.8485

>AT74444 WP_100920850.1 NZ_CP020370:c4973134-4972892 [Candidatus Thiodictyon syntrophicum]
MQPERHVRLFRNGRNQALRIPRELELDADEATIRRDGKCLMVEPVKRRPDLASLLAGWEPLDEPFPDVDQGLVPLDDIRL

Nucleotide

• Length: 243 bp

>AT74444 NZ_CP020370:c4973134-4972892 [Candidatus Thiodictyon syntrophicum]
ATGCAGCCTGAACGCCACGTCCGCCTGTTTCGCAACGGACGCAATCAAGCACTTCGCATCCCGCGCGAACTCGAACTCGA
CGCGGACGAGGCCACTATCCGCAGGGACGGCAAGTGTCTGATGGTCGAACCGGTGAAGCGCCGGCCTGACCTCGCAAGCC
TCCTTGCCGGATGGGAGCCGCTCGATGAGCCCTTCCCCGACGTGGATCAAGGTCTGGTGCCCCTGGACGACATCCGCCTC
TAA