Detailed information of TA system TA074441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2308521..2310255
• Replicon: chromosome
• Accession: NZ_CP020370
• Organism: Candidatus Thiodictyon syntrophicum strain Cad16T

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: THSYN_RS09855
• Protein ID: WP_100918986.1
• Coordinates: 2308521..2309177 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: THSYN_RS09860
• Protein ID: WP_100918987.1
• Coordinates: 2309161..2310255 (+)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THSYN_RS09845	2303922..2306627	+	2706	WP_100918984.1	type I restriction-modification system subunit M	-
THSYN_RS09850	2306633..2307931	+	1299	WP_100918985.1	restriction endonuclease subunit S	-
THSYN_RS09855	2308521..2309177	+	657	WP_100918986.1	DUF4433 domain-containing protein	Toxin
THSYN_RS09860	2309161..2310255	+	1095	WP_100918987.1	macro domain-containing protein	Antitoxin
THSYN_RS09865	2310268..2313495	+	3228	WP_100918988.1	type I restriction endonuclease subunit R	-
THSYN_RS09870	2313497..2314177	+	681	WP_100918989.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2308521..2333283	24762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (13-213)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 24904.60 Da
• Isoelectric Point: 7.6834

>T74441 WP_100918986.1 NZ_CP020370:2308521-2309177 [Candidatus Thiodictyon syntrophicum]
MSLNLNPEKALIFRIIHAQNVPWVMDHGLHCRNSAIQDSNFRTIGNPDLIDKRHQRIVPVPPGGTLSDYVPFYFTPSSPM
LYNLTTGFGGITQCPNEDIVVLVSSLPRLRQLRQCFVFTDRHAYAQAAQYFCDPADLKNIDWAILRNRDFKRDPDDPGKF
ERYQAEALVHRQVSFAALLGIVCYTDGVKLALKEQAMSRGIEIKVLCEPGWYFRDPLH

Nucleotide

• Length: 657 bp

>T74441 NZ_CP020370:2308521-2309177 [Candidatus Thiodictyon syntrophicum]
ATGTCACTGAATCTGAATCCAGAGAAGGCACTGATATTCCGAATCATTCACGCACAGAATGTGCCGTGGGTCATGGATCA
TGGCCTGCATTGCCGAAATTCCGCAATCCAAGATTCCAACTTCCGCACGATCGGGAACCCCGACCTCATTGATAAGCGGC
ATCAACGCATAGTCCCCGTACCCCCGGGCGGGACCTTGAGCGATTATGTACCCTTTTACTTTACCCCGTCATCACCCATG
TTGTACAATCTGACGACGGGCTTTGGTGGCATCACTCAATGCCCGAATGAAGACATCGTGGTCCTGGTGTCGAGCCTTCC
GCGACTGAGACAGTTGCGCCAATGCTTCGTCTTCACTGATCGCCATGCCTATGCGCAGGCGGCTCAATATTTCTGCGATC
CGGCGGATTTGAAGAACATCGATTGGGCGATACTTCGGAATCGCGATTTCAAGCGCGACCCGGATGACCCGGGTAAATTC
GAGCGTTATCAGGCGGAGGCGCTGGTTCATCGTCAGGTGTCTTTCGCCGCACTGCTGGGCATTGTCTGCTATACTGATGG
AGTCAAGTTGGCACTTAAAGAACAGGCGATGTCGCGAGGCATCGAGATCAAGGTGCTTTGTGAACCGGGCTGGTATTTCC
GTGATCCACTACACTAA

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 41113.28 Da
• Isoelectric Point: 7.2360

>AT74441 WP_100918987.1 NZ_CP020370:2309161-2310255 [Candidatus Thiodictyon syntrophicum]
VIHYTKGNLLDADVEAVVNTVNTVGVMGKGIALMFKERFPQNFSAYAAVCKDKKLAPGGIFVTERTDLLGPRWVINFATK
GHWRHPSKMEWIDTGLADLRRFIREHGVKSIAVPPLGAGNGKLDWSEVKPRIEAALGDLTEVDVIVYEPTSQYQNVAKRS
GVEKLTPARALIAELVRRYWILGIECSLLEIQKLAWFLERNIEIYALDDPLDLRFEANKYGPYARRLHHLINGLDGSYLH
CHKRLADAGSLDVIWFEDSKKEYIKVYLDGPEAKPYRAAFHETTALIDGFETPLGLELLATTDWLIDREGCPATVEGILD
GLKHWPGGGPEAGPRKLKLFDERLIRLALERRSQWDQGKFQTVR

Nucleotide

• Length: 1095 bp

>AT74441 NZ_CP020370:2309161-2310255 [Candidatus Thiodictyon syntrophicum]
GTGATCCACTACACTAAAGGCAATCTGTTGGACGCCGATGTCGAGGCGGTCGTCAATACGGTCAATACCGTGGGCGTGAT
GGGTAAAGGCATCGCGCTCATGTTCAAAGAGCGGTTTCCTCAGAATTTTTCGGCCTATGCGGCGGTCTGTAAGGACAAGA
AATTGGCGCCTGGGGGGATCTTTGTTACCGAGCGCACCGATCTGCTGGGGCCTCGCTGGGTGATCAATTTCGCCACCAAG
GGGCATTGGCGTCACCCCAGCAAGATGGAATGGATCGATACGGGTCTTGCAGATCTGCGTCGCTTCATTCGGGAACACGG
GGTGAAATCCATCGCCGTGCCGCCCCTGGGTGCCGGTAACGGCAAGCTCGATTGGTCCGAGGTCAAGCCGCGTATCGAGG
CGGCGCTGGGCGACTTGACAGAGGTTGATGTCATCGTCTATGAACCTACCAGCCAATACCAGAATGTAGCCAAACGTAGC
GGCGTAGAGAAGCTCACGCCGGCACGCGCCTTGATTGCGGAGCTGGTGCGGCGCTATTGGATACTGGGGATCGAATGCTC
GCTGCTCGAGATACAGAAGCTGGCGTGGTTCCTGGAGCGCAATATCGAGATCTATGCGCTCGACGATCCGCTGGACCTTC
GCTTTGAAGCCAATAAATACGGTCCCTATGCCCGCCGACTACATCACCTGATCAACGGTCTGGACGGAAGCTACCTGCAC
TGCCATAAGCGCCTGGCGGACGCCGGCTCCTTGGATGTCATCTGGTTCGAGGACAGCAAGAAGGAATATATCAAGGTCTA
CCTCGACGGTCCTGAGGCAAAGCCCTACCGCGCTGCCTTCCACGAAACCACCGCGTTGATCGACGGGTTCGAGACCCCTT
TGGGGTTAGAGCTTCTGGCGACAACGGATTGGCTGATCGATCGGGAAGGCTGCCCGGCGACGGTTGAGGGCATATTGGAC
GGATTGAAACACTGGCCGGGCGGCGGCCCTGAGGCTGGTCCACGCAAGCTGAAGTTGTTCGACGAACGTCTCATCCGGCT
GGCCCTAGAGCGTCGGTCCCAATGGGACCAGGGCAAGTTCCAAACAGTGCGCTGA