Detailed information of TA system TA074408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 2000246..2001228
• Replicon: chromosome
• Accession: NZ_CP020364
• Organism: Sulfolobus acidocaldarius strain DG1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IXW8
• Locus tag: B6A19_RS10655
• Protein ID: WP_011278959.1
• Coordinates: 2000608..2001228 (+)
• Length: 207 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I813
• Locus tag: B6A19_RS10650
• Protein ID: WP_011278958.1
• Coordinates: 2000246..2000596 (+)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A19_RS10630	1995650..1997584	-	1935	WP_011278954.1	DUF2079 domain-containing protein	-
B6A19_RS10635	1997793..1998497	+	705	WP_011278955.1	zinc ribbon domain-containing protein	-
B6A19_RS10640	1998581..1999225	-	645	WP_011278956.1	2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase	-
B6A19_RS10645	1999272..1999967	-	696	WP_011278957.1	glycerophosphodiester phosphodiesterase	-
B6A19_RS10650	2000246..2000596	+	351	WP_011278958.1	DUF488 family protein	Antitoxin
B6A19_RS10655	2000608..2001228	+	621	WP_011278959.1	helix-turn-helix domain-containing protein	Toxin
B6A19_RS10660	2001333..2002739	-	1407	WP_015385784.1	amidase	-
B6A19_RS10665	2002943..2003392	+	450	WP_230937910.1	hypothetical protein	-
B6A19_RS10670	2003482..2005128	-	1647	WP_011278962.1	thermopsin family protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (147-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 207 a.a.
• Molecular weight: 23490.89 Da
• Isoelectric Point: 9.6857

>T74408 WP_011278959.1 NZ_CP020364:2000608-2001228 [Sulfolobus acidocaldarius]
MGAGDVKKPINVTLILQNHPCLVMKLFEEMNVKAEINNVKMRESVTDHIATLKLTEKLLKELKERRSKTLRISENSVWIR
TEGCQVCKILYVSDAVVEKVKVVGSDAVMYKLIVPNLLSLKSLIDELNKIGVKAMVGSISELDEEQLTERQLEILKLSYK
MGFFDVDRRITMTELAEKLGIKAPTLEEILRRALRKAVKYYLDKKG

Nucleotide

• Length: 621 bp

>T74408 NZ_CP020364:2000608-2001228 [Sulfolobus acidocaldarius]
ATGGGTGCTGGTGATGTAAAGAAACCTATAAATGTTACACTTATTCTACAGAATCACCCCTGCTTAGTTATGAAATTATT
TGAAGAGATGAATGTAAAGGCGGAAATTAACAATGTGAAGATGAGGGAAAGTGTTACTGACCACATTGCAACCTTAAAGT
TAACTGAAAAGTTGCTTAAGGAGTTGAAAGAAAGAAGGTCAAAAACCTTGAGGATAAGTGAAAATAGCGTCTGGATCAGG
ACTGAAGGTTGCCAGGTATGTAAAATCCTTTATGTAAGTGACGCTGTAGTTGAGAAAGTGAAAGTTGTAGGAAGTGATGC
AGTTATGTATAAACTAATTGTTCCAAATCTACTCTCTCTGAAGAGTCTTATAGATGAGCTCAACAAGATAGGTGTAAAGG
CTATGGTAGGTAGCATATCTGAGCTAGATGAAGAGCAACTGACAGAAAGGCAATTGGAGATATTGAAATTATCATATAAG
ATGGGTTTCTTTGATGTAGACAGAAGAATAACCATGACGGAATTAGCTGAAAAACTTGGGATCAAGGCTCCTACTTTGGA
AGAGATACTGAGAAGGGCTCTACGAAAGGCAGTTAAGTATTATCTAGATAAAAAAGGATAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13918.19 Da
• Isoelectric Point: 8.2649

>AT74408 WP_011278958.1 NZ_CP020364:2000246-2000596 [Sulfolobus acidocaldarius]
MIKIKNVYDKPEINDGIRILVERTWPASVTRGSIDMWLRDLAPSEELITWYNYEPQKWEEFKRRYLEELRYNPKVKPLLM
IVKMTKIVTFLYSGDPTYNNAVVLKEYIESLINKES

Nucleotide

• Length: 351 bp

>AT74408 NZ_CP020364:2000246-2000596 [Sulfolobus acidocaldarius]
ATGATAAAAATAAAAAACGTTTATGACAAACCAGAGATTAACGACGGGATTAGAATATTGGTCGAGAGGACATGGCCAGC
CTCTGTAACTAGAGGCTCTATCGATATGTGGCTTAGGGATCTTGCCCCTTCTGAAGAATTAATAACATGGTATAATTATG
AACCGCAAAAATGGGAGGAATTTAAGAGAAGATATCTAGAAGAGTTGAGGTACAATCCAAAAGTTAAACCTTTATTGATG
ATAGTTAAGATGACTAAGATCGTAACATTTCTATACTCAGGAGACCCCACATACAATAACGCAGTTGTGCTCAAAGAATA
CATAGAAAGTTTAATAAATAAGGAAAGTTAA

Structures

Toxin

• AlphaFold DB: M1IXW8

Antitoxin

• AlphaFold DB: M1I813