Detailed information of TA system TA074403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 2012574..2013556
• Replicon: chromosome
• Accession: NZ_CP020363
• Organism: Sulfolobus acidocaldarius strain Y14 13-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IXW8
• Locus tag: B6A20_RS10750
• Protein ID: WP_011278959.1
• Coordinates: 2012936..2013556 (+)
• Length: 207 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I813
• Locus tag: B6A20_RS10745
• Protein ID: WP_011278958.1
• Coordinates: 2012574..2012924 (+)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A20_RS10725	2007978..2009912	-	1935	WP_011278954.1	DUF2079 domain-containing protein	-
B6A20_RS10730	2010121..2010825	+	705	WP_011278955.1	zinc ribbon domain-containing protein	-
B6A20_RS10735	2010909..2011553	-	645	WP_011278956.1	2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase	-
B6A20_RS10740	2011600..2012295	-	696	WP_011278957.1	glycerophosphodiester phosphodiesterase	-
B6A20_RS10745	2012574..2012924	+	351	WP_011278958.1	DUF488 family protein	Antitoxin
B6A20_RS10750	2012936..2013556	+	621	WP_011278959.1	helix-turn-helix domain-containing protein	Toxin
B6A20_RS10755	2013661..2015067	-	1407	WP_015385784.1	amidase	-
B6A20_RS10760	2015271..2015720	+	450	WP_230937910.1	hypothetical protein	-
B6A20_RS10765	2015810..2017456	-	1647	WP_011278962.1	thermopsin family protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (147-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 207 a.a.
• Molecular weight: 23490.89 Da
• Isoelectric Point: 9.6857

>T74403 WP_011278959.1 NZ_CP020363:2012936-2013556 [Sulfolobus acidocaldarius]
MGAGDVKKPINVTLILQNHPCLVMKLFEEMNVKAEINNVKMRESVTDHIATLKLTEKLLKELKERRSKTLRISENSVWIR
TEGCQVCKILYVSDAVVEKVKVVGSDAVMYKLIVPNLLSLKSLIDELNKIGVKAMVGSISELDEEQLTERQLEILKLSYK
MGFFDVDRRITMTELAEKLGIKAPTLEEILRRALRKAVKYYLDKKG

Nucleotide

• Length: 621 bp

>T74403 NZ_CP020363:2012936-2013556 [Sulfolobus acidocaldarius]
ATGGGTGCTGGTGATGTAAAGAAACCTATAAATGTTACACTTATTCTACAGAATCACCCCTGCTTAGTTATGAAATTATT
TGAAGAGATGAATGTAAAGGCGGAAATTAACAATGTGAAGATGAGGGAAAGTGTTACTGACCACATTGCAACCTTAAAGT
TAACTGAAAAGTTGCTTAAGGAGTTGAAAGAAAGAAGGTCAAAAACCTTGAGGATAAGTGAAAATAGCGTCTGGATCAGG
ACTGAAGGTTGCCAGGTATGTAAAATCCTTTATGTAAGTGACGCTGTAGTTGAGAAAGTGAAAGTTGTAGGAAGTGATGC
AGTTATGTATAAACTAATTGTTCCAAATCTACTCTCTCTGAAGAGTCTTATAGATGAGCTCAACAAGATAGGTGTAAAGG
CTATGGTAGGTAGCATATCTGAGCTAGATGAAGAGCAACTGACAGAAAGGCAATTGGAGATATTGAAATTATCATATAAG
ATGGGTTTCTTTGATGTAGACAGAAGAATAACCATGACGGAATTAGCTGAAAAACTTGGGATCAAGGCTCCTACTTTGGA
AGAGATACTGAGAAGGGCTCTACGAAAGGCAGTTAAGTATTATCTAGATAAAAAAGGATAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13918.19 Da
• Isoelectric Point: 8.2649

>AT74403 WP_011278958.1 NZ_CP020363:2012574-2012924 [Sulfolobus acidocaldarius]
MIKIKNVYDKPEINDGIRILVERTWPASVTRGSIDMWLRDLAPSEELITWYNYEPQKWEEFKRRYLEELRYNPKVKPLLM
IVKMTKIVTFLYSGDPTYNNAVVLKEYIESLINKES

Nucleotide

• Length: 351 bp

>AT74403 NZ_CP020363:2012574-2012924 [Sulfolobus acidocaldarius]
ATGATAAAAATAAAAAACGTTTATGACAAACCAGAGATTAACGACGGGATTAGAATATTGGTCGAGAGGACATGGCCAGC
CTCTGTAACTAGAGGCTCTATCGATATGTGGCTTAGGGATCTTGCCCCTTCTGAAGAATTAATAACATGGTATAATTATG
AACCGCAAAAATGGGAGGAATTTAAGAGAAGATATCTAGAAGAGTTGAGGTACAATCCAAAAGTTAAACCTTTATTGATG
ATAGTTAAGATGACTAAGATCGTAACATTTCTATACTCAGGAGACCCCACATACAATAACGCAGTTGTGCTCAAAGAATA
CATAGAAAGTTTAATAAATAAGGAAAGTTAA

Structures

Toxin

• AlphaFold DB: M1IXW8

Antitoxin

• AlphaFold DB: M1I813