Detailed information of TA system TA074401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1761747..1762353
• Replicon: chromosome
• Accession: NZ_CP020363
• Organism: Sulfolobus acidocaldarius strain Y14 13-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1ITB5
• Locus tag: B6A20_RS09550
• Protein ID: WP_011278759.1
• Coordinates: 1761747..1762142 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I7B9
• Locus tag: B6A20_RS09555
• Protein ID: WP_011278760.1
• Coordinates: 1762126..1762353 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A20_RS09525	1758099..1758629	+	531	WP_011278754.1	PaREP1 family protein	-
B6A20_RS09530	1759238..1759915	-	678	WP_011278755.1	hypothetical protein	-
B6A20_RS12330	1760514..1760639	-	126	WP_257719735.1	hypothetical protein	-
B6A20_RS09540	1760878..1761126	+	249	WP_011278757.1	hypothetical protein	-
B6A20_RS09545	1761119..1761538	+	420	WP_058692945.1	PIN domain-containing protein	-
B6A20_RS09550	1761747..1762142	-	396	WP_011278759.1	PIN domain-containing protein	Toxin
B6A20_RS09555	1762126..1762353	-	228	WP_011278760.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B6A20_RS09560	1762453..1762896	-	444	WP_011278761.1	MarR family transcriptional regulator	-
B6A20_RS09565	1762883..1764586	-	1704	WP_011278762.1	MFS transporter	-
B6A20_RS09570	1765163..1765660	+	498	WP_011278763.1	hypothetical protein	-
B6A20_RS09575	1765681..1766352	+	672	WP_015385737.1	hypothetical protein	-
B6A20_RS12075	1766358..1767125	+	768	WP_011278765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (3-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15289.79 Da
• Isoelectric Point: 5.7030

>T74401 WP_011278759.1 NZ_CP020363:c1762142-1761747 [Sulfolobus acidocaldarius]
MKILVDTNVLIYDLVENSKFHKEAEDILDRAETWVIPSVVVHELVWFLRANNLERMDYVSAYVESPKAEIVCEDFDVLKN
AMEIILREKISLSRYNDPVILSHAILRKLPLSTFDRRLSNLAKRYGIDIVK

Nucleotide

• Length: 396 bp

>T74401 NZ_CP020363:c1762142-1761747 [Sulfolobus acidocaldarius]
ATGAAAATTTTAGTTGATACTAACGTACTAATCTATGACCTCGTGGAGAACTCAAAGTTTCACAAGGAAGCTGAGGATAT
ATTGGACAGAGCAGAAACTTGGGTCATACCTTCTGTAGTCGTTCATGAGCTAGTATGGTTCTTGAGGGCGAATAACCTGG
AGAGAATGGACTACGTATCAGCATATGTGGAAAGCCCCAAGGCTGAAATAGTGTGCGAGGACTTTGATGTTTTAAAGAAC
GCAATGGAAATAATACTGAGGGAAAAGATATCTCTTTCTAGGTATAATGACCCAGTAATACTCTCACACGCGATTCTCAG
GAAGTTACCACTTTCGACGTTTGATAGGAGACTCTCCAACTTGGCAAAGAGGTACGGAATAGACATAGTTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8808.27 Da
• Isoelectric Point: 8.4634

>AT74401 WP_011278760.1 NZ_CP020363:c1762353-1762126 [Sulfolobus acidocaldarius]
VEKVKVTRNYQVTIPSSVRNVLGIKEGDILEVYVENDKIVMKKVESKRPRIKIEKLMTIEYIEESIERGMHENFS

Nucleotide

• Length: 228 bp

>AT74401 NZ_CP020363:c1762353-1762126 [Sulfolobus acidocaldarius]
GTGGAGAAGGTAAAAGTTACTAGGAACTACCAGGTCACGATACCTTCATCTGTGAGGAACGTCCTAGGAATTAAGGAGGG
TGATATTTTGGAGGTGTACGTAGAGAATGATAAAATAGTGATGAAGAAGGTAGAGTCTAAGAGACCTAGGATAAAGATTG
AAAAACTTATGACCATAGAATATATAGAGGAAAGTATAGAGAGAGGCATGCATGAAAATTTTAGTTGA

Structures

Toxin

• AlphaFold DB: M1ITB5

Antitoxin

• AlphaFold DB: M1I7B9