Detailed information of TA system TA074395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1721140..1721749
• Replicon: chromosome
• Accession: NZ_CP020362
• Organism: Sulfolobus acidocaldarius strain Y14 16-22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IXF9
• Locus tag: B6A17_RS09485
• Protein ID: WP_011278690.1
• Coordinates: 1721354..1721749 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1J7H5
• Locus tag: B6A17_RS09480
• Protein ID: WP_011278689.1
• Coordinates: 1721140..1721364 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A17_RS09480	1721140..1721364	+	225	WP_011278689.1	hypothetical protein	Antitoxin
B6A17_RS09485	1721354..1721749	+	396	WP_011278690.1	twitching motility protein PilT	Toxin
B6A17_RS09490	1722231..1723121	-	891	WP_015385703.1	DUF1177 domain-containing protein	-
B6A17_RS09495	1723118..1724173	-	1056	WP_232463147.1	FAD-binding oxidoreductase	-
B6A17_RS12575	1724163..1724393	+	231	WP_232463148.1	hypothetical protein	-
B6A17_RS09500	1724636..1724890	-	255	WP_015385704.1	hypothetical protein	-
B6A17_RS12385	1725159..1726349	-	1191	WP_011278693.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (8-116)

Antitoxin

• Putative antitoxin VapB3-like (1-74)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14959.46 Da
• Isoelectric Point: 5.7407

>T74395 WP_011278690.1 NZ_CP020362:1721354-1721749 [Sulfolobus acidocaldarius]
MRGKFLFDASAIYPLIKFVDKVDLKNIYILHLTLYEVGNAIWKDAFLHGRIKNAKEVALLFQQLMGEFNIIDDPPLDEVI
GIAISKGITYYDASYVYVSAKEKLILVSNDKELISKGNTISFNEFMEKYLA

Nucleotide

• Length: 396 bp

>T74395 NZ_CP020362:1721354-1721749 [Sulfolobus acidocaldarius]
ATGAGAGGTAAATTCCTTTTTGACGCTTCAGCAATATACCCCTTAATCAAATTCGTTGATAAGGTGGACCTAAAGAACAT
TTACATACTACATTTAACTTTATATGAGGTAGGGAATGCAATATGGAAAGATGCTTTTCTACACGGGAGGATTAAGAACG
CTAAAGAAGTAGCCCTCCTCTTCCAACAGTTAATGGGAGAGTTTAACATCATCGACGACCCTCCTCTGGACGAGGTCATA
GGAATAGCTATAAGTAAGGGCATTACATATTACGATGCTTCTTATGTCTACGTCTCTGCTAAGGAGAAATTGATATTAGT
GAGCAACGACAAAGAGCTCATCTCTAAAGGTAATACTATATCCTTTAACGAGTTTATGGAAAAATATCTCGCCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8878.21 Da
• Isoelectric Point: 8.7436

>AT74395 WP_011278689.1 NZ_CP020362:1721140-1721364 [Sulfolobus acidocaldarius]
MSDVISVRVRKELKRRAEELGINLREVVERALEEAIRKKEMERVRTIAKKIQENMQGISEEEWAQLVRESRDER

Nucleotide

• Length: 225 bp

>AT74395 NZ_CP020362:1721140-1721364 [Sulfolobus acidocaldarius]
ATGTCAGACGTAATTAGTGTAAGAGTAAGAAAAGAATTGAAGAGGAGGGCTGAGGAGTTGGGAATTAACCTGAGGGAAGT
TGTGGAGAGGGCACTTGAGGAGGCTATAAGGAAAAAAGAGATGGAGAGGGTTAGGACTATAGCTAAAAAGATCCAGGAAA
ATATGCAGGGTATAAGTGAGGAGGAGTGGGCTCAATTAGTCAGGGAGAGTAGGGATGAGAGGTAA

Structures

Toxin

• AlphaFold DB: M1IXF9

Antitoxin

• AlphaFold DB: M1J7H5