Detailed information of TA system TA074394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 2063699..2064686
• Replicon: chromosome
• Accession: NZ_CP020361
• Organism: Sulfolobus acidocaldarius strain Y14 20-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IU00
• Locus tag: B6A16_RS11015
• Protein ID: WP_011278971.1
• Coordinates: 2063699..2064316 (-)
• Length: 206 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I835
• Locus tag: B6A16_RS11020
• Protein ID: WP_011278972.1
• Coordinates: 2064342..2064686 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A16_RS11000	2059302..2059904	+	603	WP_011278968.1	hypothetical protein	-
B6A16_RS11005	2059956..2061347	-	1392	WP_011278969.1	APC family permease	-
B6A16_RS11010	2061498..2063492	+	1995	WP_011278970.1	primary-amine oxidase	-
B6A16_RS11015	2063699..2064316	-	618	WP_011278971.1	helix-turn-helix domain-containing protein	Toxin
B6A16_RS11020	2064342..2064686	-	345	WP_011278972.1	DUF488 domain-containing protein	Antitoxin
B6A16_RS11025	2064992..2065780	+	789	WP_011278973.1	aldose 1-epimerase	-
B6A16_RS11030	2066078..2067106	+	1029	WP_088899929.1	NAD-binding protein	-
B6A16_RS11035	2067694..2068323	-	630	WP_011278975.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (146-196)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 206 a.a.
• Molecular weight: 23779.04 Da
• Isoelectric Point: 9.9689

>T74394 WP_011278971.1 NZ_CP020361:c2064316-2063699 [Sulfolobus acidocaldarius]
MVFASKRPIEVTLLVDKHPCEVLDIFRKYEIKAQITNVKLRDQVTDHIAFLKINDDILKELKRRSLKTLRINENTIWIRT
NGCGVCKLLYHSDLIVEKVKVLSKATVMYKLLLPNLSALKQFLEELNSIRVEATVANVSEIDESELTDRQLEILRLAYKS
GYFDVDRKISMKELANKLGIKASTLEEILRRALKKAVKYYLDRKS

Nucleotide

• Length: 618 bp

>T74394 NZ_CP020361:c2064316-2063699 [Sulfolobus acidocaldarius]
ATGGTCTTTGCCTCAAAGAGACCCATTGAAGTTACACTCCTAGTGGATAAACACCCATGCGAAGTCTTAGACATATTTAG
AAAATATGAAATTAAGGCTCAGATAACTAATGTTAAGCTAAGGGATCAGGTTACTGACCATATTGCATTCCTTAAGATAA
ACGATGATATACTCAAGGAATTAAAGAGAAGGTCTCTTAAAACTTTAAGGATAAATGAAAATACCATTTGGATAAGGACA
AACGGTTGCGGCGTTTGTAAACTACTCTATCACTCTGACCTAATTGTGGAAAAGGTAAAGGTGTTAAGTAAAGCTACTGT
TATGTATAAACTTCTATTACCAAATCTTTCTGCATTGAAACAGTTTTTAGAAGAACTGAACAGCATAAGAGTAGAGGCTA
CAGTTGCCAATGTATCAGAAATTGACGAGAGCGAGTTAACAGATAGACAATTGGAAATCCTGAGATTGGCTTACAAATCA
GGCTATTTTGATGTCGATAGGAAAATCTCGATGAAGGAATTAGCCAATAAATTGGGGATAAAGGCATCTACGCTAGAGGA
GATACTGAGGAGGGCTCTAAAAAAGGCTGTAAAATATTATTTGGACAGAAAGAGCTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13706.92 Da
• Isoelectric Point: 9.2564

>AT74394 WP_011278972.1 NZ_CP020361:c2064686-2064342 [Sulfolobus acidocaldarius]
MIKVKRVYDAPESDDGVRILIDRLWPRGVKKDLVDIWMKDIAPSDELRKWFNHEPEKWEEFKHKYMEELRSNPKVKVLIE
VIRRSENVTFLYATKSPYNNAVVLKEYIESLLTK

Nucleotide

• Length: 345 bp

>AT74394 NZ_CP020361:c2064686-2064342 [Sulfolobus acidocaldarius]
ATGATTAAGGTCAAGCGAGTTTATGACGCACCTGAATCTGATGATGGGGTCAGAATATTGATTGACCGATTATGGCCTAG
AGGAGTAAAGAAAGATCTAGTGGATATTTGGATGAAAGATATTGCTCCATCAGATGAGTTAAGGAAATGGTTTAATCATG
AACCTGAAAAGTGGGAAGAGTTTAAGCACAAGTATATGGAGGAGCTTAGAAGTAACCCTAAGGTCAAAGTCTTAATCGAG
GTAATAAGGAGAAGCGAAAATGTTACATTCCTATATGCCACTAAATCGCCTTACAATAATGCGGTAGTTTTAAAGGAGTA
TATTGAAAGTTTGTTAACAAAATAA

Structures

Toxin

• AlphaFold DB: M1IU00

Antitoxin

• AlphaFold DB: M1I835