Detailed information of TA system TA074389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 1964954..1965941
• Replicon: chromosome
• Accession: NZ_CP020360
• Organism: Sulfolobus acidocaldarius strain Y14 18-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IU00
• Locus tag: B6A18_RS10680
• Protein ID: WP_011278971.1
• Coordinates: 1964954..1965571 (-)
• Length: 206 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I835
• Locus tag: B6A18_RS10685
• Protein ID: WP_011278972.1
• Coordinates: 1965597..1965941 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A18_RS10665	1960557..1961159	+	603	WP_011278968.1	hypothetical protein	-
B6A18_RS10670	1961211..1962602	-	1392	WP_011278969.1	APC family permease	-
B6A18_RS10675	1962753..1964747	+	1995	WP_011278970.1	primary-amine oxidase	-
B6A18_RS10680	1964954..1965571	-	618	WP_011278971.1	helix-turn-helix domain-containing protein	Toxin
B6A18_RS10685	1965597..1965941	-	345	WP_011278972.1	DUF488 domain-containing protein	Antitoxin
B6A18_RS10690	1966247..1967035	+	789	WP_011278973.1	aldose 1-epimerase	-
B6A18_RS10695	1967333..1968361	+	1029	WP_088899929.1	NAD-binding protein	-
B6A18_RS10700	1968949..1969578	-	630	WP_011278975.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (146-196)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 206 a.a.
• Molecular weight: 23779.04 Da
• Isoelectric Point: 9.9689

>T74389 WP_011278971.1 NZ_CP020360:c1965571-1964954 [Sulfolobus acidocaldarius]
MVFASKRPIEVTLLVDKHPCEVLDIFRKYEIKAQITNVKLRDQVTDHIAFLKINDDILKELKRRSLKTLRINENTIWIRT
NGCGVCKLLYHSDLIVEKVKVLSKATVMYKLLLPNLSALKQFLEELNSIRVEATVANVSEIDESELTDRQLEILRLAYKS
GYFDVDRKISMKELANKLGIKASTLEEILRRALKKAVKYYLDRKS

Nucleotide

• Length: 618 bp

>T74389 NZ_CP020360:c1965571-1964954 [Sulfolobus acidocaldarius]
ATGGTCTTTGCCTCAAAGAGACCCATTGAAGTTACACTCCTAGTGGATAAACACCCATGCGAAGTCTTAGACATATTTAG
AAAATATGAAATTAAGGCTCAGATAACTAATGTTAAGCTAAGGGATCAGGTTACTGACCATATTGCATTCCTTAAGATAA
ACGATGATATACTCAAGGAATTAAAGAGAAGGTCTCTTAAAACTTTAAGGATAAATGAAAATACCATTTGGATAAGGACA
AACGGTTGCGGCGTTTGTAAACTACTCTATCACTCTGACCTAATTGTGGAAAAGGTAAAGGTGTTAAGTAAAGCTACTGT
TATGTATAAACTTCTATTACCAAATCTTTCTGCATTGAAACAGTTTTTAGAAGAACTGAACAGCATAAGAGTAGAGGCTA
CAGTTGCCAATGTATCAGAAATTGACGAGAGCGAGTTAACAGATAGACAATTGGAAATCCTGAGATTGGCTTACAAATCA
GGCTATTTTGATGTCGATAGGAAAATCTCGATGAAGGAATTAGCCAATAAATTGGGGATAAAGGCATCTACGCTAGAGGA
GATACTGAGGAGGGCTCTAAAAAAGGCTGTAAAATATTATTTGGACAGAAAGAGCTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13706.92 Da
• Isoelectric Point: 9.2564

>AT74389 WP_011278972.1 NZ_CP020360:c1965941-1965597 [Sulfolobus acidocaldarius]
MIKVKRVYDAPESDDGVRILIDRLWPRGVKKDLVDIWMKDIAPSDELRKWFNHEPEKWEEFKHKYMEELRSNPKVKVLIE
VIRRSENVTFLYATKSPYNNAVVLKEYIESLLTK

Nucleotide

• Length: 345 bp

>AT74389 NZ_CP020360:c1965941-1965597 [Sulfolobus acidocaldarius]
ATGATTAAGGTCAAGCGAGTTTATGACGCACCTGAATCTGATGATGGGGTCAGAATATTGATTGACCGATTATGGCCTAG
AGGAGTAAAGAAAGATCTAGTGGATATTTGGATGAAAGATATTGCTCCATCAGATGAGTTAAGGAAATGGTTTAATCATG
AACCTGAAAAGTGGGAAGAGTTTAAGCACAAGTATATGGAGGAGCTTAGAAGTAACCCTAAGGTCAAAGTCTTAATCGAG
GTAATAAGGAGAAGCGAAAATGTTACATTCCTATATGCCACTAAATCGCCTTACAATAATGCGGTAGTTTTAAAGGAGTA
TATTGAAAGTTTGTTAACAAAATAA

Structures

Toxin

• AlphaFold DB: M1IU00

Antitoxin

• AlphaFold DB: M1I835