Detailed information of TA system TA074386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1695221..1695827
• Replicon: chromosome
• Accession: NZ_CP020360
• Organism: Sulfolobus acidocaldarius strain Y14 18-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1ITB5
• Locus tag: B6A18_RS09405
• Protein ID: WP_011278759.1
• Coordinates: 1695221..1695616 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I7B9
• Locus tag: B6A18_RS09410
• Protein ID: WP_011278760.1
• Coordinates: 1695600..1695827 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6A18_RS09380	1691573..1692103	+	531	WP_011278754.1	PaREP1 family protein	-
B6A18_RS09385	1692712..1693389	-	678	WP_011278755.1	hypothetical protein	-
B6A18_RS12150	1693988..1694113	-	126	WP_257719735.1	hypothetical protein	-
B6A18_RS09395	1694352..1694600	+	249	WP_011278757.1	hypothetical protein	-
B6A18_RS09400	1694593..1695012	+	420	WP_058692945.1	PIN domain-containing protein	-
B6A18_RS09405	1695221..1695616	-	396	WP_011278759.1	PIN domain-containing protein	Toxin
B6A18_RS09410	1695600..1695827	-	228	WP_011278760.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B6A18_RS09415	1695927..1696370	-	444	WP_011278761.1	MarR family transcriptional regulator	-
B6A18_RS09420	1696357..1698060	-	1704	WP_011278762.1	MFS transporter	-
B6A18_RS09425	1698637..1699134	+	498	WP_011278763.1	hypothetical protein	-
B6A18_RS09430	1699155..1699826	+	672	WP_015385737.1	hypothetical protein	-
B6A18_RS11925	1699832..1700599	+	768	WP_011278765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (3-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15289.79 Da
• Isoelectric Point: 5.7030

>T74386 WP_011278759.1 NZ_CP020360:c1695616-1695221 [Sulfolobus acidocaldarius]
MKILVDTNVLIYDLVENSKFHKEAEDILDRAETWVIPSVVVHELVWFLRANNLERMDYVSAYVESPKAEIVCEDFDVLKN
AMEIILREKISLSRYNDPVILSHAILRKLPLSTFDRRLSNLAKRYGIDIVK

Nucleotide

• Length: 396 bp

>T74386 NZ_CP020360:c1695616-1695221 [Sulfolobus acidocaldarius]
ATGAAAATTTTAGTTGATACTAACGTACTAATCTATGACCTCGTGGAGAACTCAAAGTTTCACAAGGAAGCTGAGGATAT
ATTGGACAGAGCAGAAACTTGGGTCATACCTTCTGTAGTCGTTCATGAGCTAGTATGGTTCTTGAGGGCGAATAACCTGG
AGAGAATGGACTACGTATCAGCATATGTGGAAAGCCCCAAGGCTGAAATAGTGTGCGAGGACTTTGATGTTTTAAAGAAC
GCAATGGAAATAATACTGAGGGAAAAGATATCTCTTTCTAGGTATAATGACCCAGTAATACTCTCACACGCGATTCTCAG
GAAGTTACCACTTTCGACGTTTGATAGGAGACTCTCCAACTTGGCAAAGAGGTACGGAATAGACATAGTTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8808.27 Da
• Isoelectric Point: 8.4634

>AT74386 WP_011278760.1 NZ_CP020360:c1695827-1695600 [Sulfolobus acidocaldarius]
VEKVKVTRNYQVTIPSSVRNVLGIKEGDILEVYVENDKIVMKKVESKRPRIKIEKLMTIEYIEESIERGMHENFS

Nucleotide

• Length: 228 bp

>AT74386 NZ_CP020360:c1695827-1695600 [Sulfolobus acidocaldarius]
GTGGAGAAGGTAAAAGTTACTAGGAACTACCAGGTCACGATACCTTCATCTGTGAGGAACGTCCTAGGAATTAAGGAGGG
TGATATTTTGGAGGTGTACGTAGAGAATGATAAAATAGTGATGAAGAAGGTAGAGTCTAAGAGACCTAGGATAAAGATTG
AAAAACTTATGACCATAGAATATATAGAGGAAAGTATAGAGAGAGGCATGCATGAAAATTTTAGTTGA

Structures

Toxin

• AlphaFold DB: M1ITB5

Antitoxin

• AlphaFold DB: M1I7B9