Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5054583..5055202 Replicon chromosome
Accession NZ_CP020358
Organism Klebsiella oxytoca strain AR_0147

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag AM394_RS24580 Protein ID WP_004099646.1
Coordinates 5054583..5054801 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag AM394_RS24585 Protein ID WP_025107145.1
Coordinates 5054828..5055202 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM394_RS24550 5050628..5050891 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
AM394_RS24555 5050891..5051031 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM394_RS24560 5051028..5051726 - 699 WP_032748285.1 GNAT family N-acetyltransferase -
AM394_RS24565 5051828..5053282 + 1455 WP_032748288.1 PLP-dependent aminotransferase family protein -
AM394_RS24570 5053257..5053727 - 471 WP_014228205.1 YlaC family protein -
AM394_RS24575 5053854..5054420 - 567 WP_032748289.1 maltose O-acetyltransferase -
AM394_RS24580 5054583..5054801 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
AM394_RS24585 5054828..5055202 - 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
AM394_RS24590 5055684..5058830 - 3147 WP_032748290.1 multidrug efflux RND transporter permease subunit -
AM394_RS24595 5058853..5060046 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T74385 WP_004099646.1 NZ_CP020358:c5054801-5054583 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T74385 NZ_CP020358:c5054801-5054583 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT74385 WP_025107145.1 NZ_CP020358:c5055202-5054828 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT74385 NZ_CP020358:c5055202-5054828 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References