Detailed information of TA system TA074383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4368951..4369527
• Replicon: chromosome
• Accession: NZ_CP020358
• Organism: Klebsiella oxytoca strain AR_0147

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: AM394_RS21355
• Protein ID: WP_080528481.1
• Coordinates: 4368951..4369238 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: AM394_RS21360
• Protein ID: WP_014227757.1
• Coordinates: 4369225..4369527 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM394_RS21325	4364411..4364929	-	519	WP_080528480.1	hypothetical protein	-
AM394_RS21330	4364926..4365726	-	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
AM394_RS21335	4365744..4366328	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
AM394_RS21340	4366476..4367258	-	783	WP_025108473.1	NAD(P)H-dependent oxidoreductase	-
AM394_RS21345	4367346..4368254	+	909	WP_049080873.1	LysR family transcriptional regulator	-
AM394_RS21350	4368248..4368691	+	444	WP_014227755.1	FosA family fosfomycin resistance glutathione transferase	-
AM394_RS21355	4368951..4369238	+	288	WP_080528481.1	BrnT family toxin	Toxin
AM394_RS21360	4369225..4369527	+	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
AM394_RS21365	4369648..4370991	-	1344	WP_049101852.1	APC family permease	-
AM394_RS21370	4370988..4371899	-	912	WP_080528482.1	acetamidase/formamidase family protein	-
AM394_RS21375	4372353..4372688	-	336	WP_080528483.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11104.61 Da
• Isoelectric Point: 8.5901

>T74383 WP_080528481.1 NZ_CP020358:4368951-4369238 [Klebsiella oxytoca]
MPMEFEWDANKAISNLRKHGIRFEEAVLVLDDPRHLSRQDRYENGAYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T74383 NZ_CP020358:4368951-4369238 [Klebsiella oxytoca]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAATAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTAGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGCATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT74383 WP_014227757.1 NZ_CP020358:4369225-4369527 [Klebsiella oxytoca]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT74383 NZ_CP020358:4369225-4369527 [Klebsiella oxytoca]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591