Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3539940..3540487 Replicon chromosome
Accession NZ_CP020358
Organism Klebsiella oxytoca strain AR_0147

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0J2H3H5
Locus tag AM394_RS17395 Protein ID WP_032751830.1
Coordinates 3540179..3540487 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag AM394_RS17390 Protein ID WP_014227298.1
Coordinates 3539940..3540176 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM394_RS17375 3536147..3537313 + 1167 WP_014227296.1 multidrug effflux MFS transporter -
AM394_RS17380 3537474..3538376 + 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
AM394_RS17385 3538401..3539834 + 1434 WP_032751833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
AM394_RS17390 3539940..3540176 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM394_RS17395 3540179..3540487 + 309 WP_032751830.1 CcdB family protein Toxin
AM394_RS17400 3540638..3541540 + 903 WP_009654083.1 DMT family transporter -
AM394_RS17405 3541544..3542497 - 954 WP_032751828.1 Rpn family recombination-promoting nuclease/putative transposase -
AM394_RS17410 3542707..3543000 + 294 WP_047722799.1 hypothetical protein -
AM394_RS17415 3543024..3543965 - 942 WP_014227303.1 LacI family transcriptional regulator -
AM394_RS17420 3544260..3544571 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11682.54 Da        Isoelectric Point: 6.7155

>T74382 WP_032751830.1 NZ_CP020358:3540179-3540487 [Klebsiella oxytoca]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQLEVVKASMDFLFDGI

Download         Length: 309 bp

>T74382 NZ_CP020358:3540179-3540487 [Klebsiella oxytoca]
ATGCAGTATTACGTATATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAG
GTTTGTAATCTGAATCACCAGCTCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT74382 WP_014227298.1 NZ_CP020358:3539940-3540176 [Klebsiella oxytoca]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT74382 NZ_CP020358:3539940-3540176 [Klebsiella oxytoca]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2H3H5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References