Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2817509..2818284 Replicon chromosome
Accession NZ_CP020358
Organism Klebsiella oxytoca strain AR_0147

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A0J2HI49
Locus tag AM394_RS13845 Protein ID WP_032751262.1
Coordinates 2817509..2817994 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0J2HGF0
Locus tag AM394_RS13850 Protein ID WP_032751264.1
Coordinates 2817991..2818284 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM394_RS13835 2816545..2816796 + 252 WP_071846062.1 hypothetical protein -
AM394_RS13845 2817509..2817994 - 486 WP_032751262.1 GNAT family N-acetyltransferase Toxin
AM394_RS13850 2817991..2818284 - 294 WP_032751264.1 DUF1778 domain-containing protein Antitoxin
AM394_RS13855 2818663..2820051 - 1389 WP_032751266.1 DASS family sodium-coupled anion symporter -
AM394_RS13860 2820597..2821967 + 1371 WP_047722911.1 cystathionine beta-synthase -
AM394_RS13865 2821978..2823123 + 1146 WP_080528379.1 PLP-dependent aspartate aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-143)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17523.45 Da        Isoelectric Point: 8.1567

>T74381 WP_032751262.1 NZ_CP020358:c2817994-2817509 [Klebsiella oxytoca]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQLSGASRTFVCCHDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDRSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLVN
A

Download         Length: 486 bp

>T74381 NZ_CP020358:c2817994-2817509 [Klebsiella oxytoca]
ATGATCTCCGCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAACCAGCTTTCTGGCGCGTCCCGTACGTTTGTCTGCTGCCACGATGCTAAGGTGATGG
CTTACTACTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGCCGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTTGTAGTGCTGGGACGTCTGGCGGTGGATCGGTCGCTACATGGACAAGGCGTTGGGCGGGCGCTGGTGAGGGATGC
CGGACTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGCGGG
AGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10960.59 Da        Isoelectric Point: 9.9171

>AT74381 WP_032751264.1 NZ_CP020358:c2818284-2817991 [Klebsiella oxytoca]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQTPAPN
AALRKTMQTPAPWEQEK

Download         Length: 294 bp

>AT74381 NZ_CP020358:c2818284-2817991 [Klebsiella oxytoca]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACTCTGAACCTGCGCATCAGGCCCGCTGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGTTGA
TTGAGCAACGCATTATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTGGCTCGCCTGGATCAGACTCCTGCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2HI49

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2HGF0

References