Detailed information of TA system TA074371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 523078..523721
• Replicon: chromosome
• Accession: NZ_CP020358
• Organism: Klebsiella oxytoca strain AR_0147

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: AM394_RS02555
• Protein ID: WP_040225651.1
• Coordinates: 523078..523494 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: AM394_RS02560
• Protein ID: WP_001261276.1
• Coordinates: 523491..523721 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM394_RS31515	518964..520616	+	1653	WP_115657786.1	L-tyrosine/L-tryptophan isonitrile synthase family protein	-
AM394_RS02535	520682..521917	+	1236	WP_048292257.1	MFS transporter	-
AM394_RS02540	522017..522410	-	394	Protein_483	CAAX protease	-
AM394_RS02545	522481..522735	-	255	Protein_484	hypothetical protein	-
AM394_RS02550	522799..523044	-	246	WP_040225648.1	hypothetical protein	-
AM394_RS02555	523078..523494	-	417	WP_040225651.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM394_RS02560	523491..523721	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM394_RS02570	524258..524572	+	315	WP_001302628.1	hypothetical protein	-
AM394_RS02575	524625..525410	+	786	WP_048292255.1	hypothetical protein	-
AM394_RS02580	525486..525863	+	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
AM394_RS02585	525860..526207	+	348	WP_048292253.1	IS66 family insertion sequence element accessory protein TnpB	-
AM394_RS02590	526257..527795	+	1539	WP_048292251.1	IS66 family transposase	-
AM394_RS02595	528249..528653	-	405	WP_048292250.1	DUF2251 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15089.57 Da
• Isoelectric Point: 8.5464

>T74371 WP_040225651.1 NZ_CP020358:c523494-523078 [Klebsiella oxytoca]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIHLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T74371 NZ_CP020358:c523494-523078 [Klebsiella oxytoca]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCATCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCGGGCCTGGTGCTTG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT74371 WP_001261276.1 NZ_CP020358:c523721-523491 [Klebsiella oxytoca]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT74371 NZ_CP020358:c523721-523491 [Klebsiella oxytoca]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023