Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 478199..478935 Replicon chromosome
Accession NZ_CP020358
Organism Klebsiella oxytoca strain AR_0147

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag AM394_RS02315 Protein ID WP_004098919.1
Coordinates 478199..478681 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag AM394_RS02320 Protein ID WP_004098921.1
Coordinates 478669..478935 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM394_RS02290 473589..473894 + 306 WP_012540235.1 PTS sugar transporter subunit IIB -
AM394_RS02295 473914..475248 + 1335 WP_049594012.1 PTS sugar transporter subunit IIC -
AM394_RS02300 475287..476666 + 1380 WP_049594011.1 glycoside hydrolase family 1 protein -
AM394_RS02305 476677..476988 + 312 WP_049594032.1 PTS lactose/cellobiose transporter subunit IIA -
AM394_RS02310 477369..477983 + 615 Protein_439 Mig-14 family protein -
AM394_RS02315 478199..478681 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
AM394_RS02320 478669..478935 - 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
AM394_RS02325 479111..479365 - 255 WP_004098924.1 hypothetical protein -
AM394_RS02330 479441..479698 - 258 WP_004098928.1 hypothetical protein -
AM394_RS02335 479747..479950 - 204 WP_004098931.1 hemolysin expression modulator Hha -
AM394_RS02340 480288..480722 - 435 WP_004098933.1 hypothetical protein -
AM394_RS02345 480938..482338 - 1401 WP_004098935.1 copper resistance membrane spanning protein PcoS -
AM394_RS02350 482335..483015 - 681 WP_004098938.1 copper response regulator transcription factor PcoR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T74370 WP_004098919.1 NZ_CP020358:c478681-478199 [Klebsiella oxytoca]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T74370 NZ_CP020358:c478681-478199 [Klebsiella oxytoca]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT74370 WP_004098921.1 NZ_CP020358:c478935-478669 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT74370 NZ_CP020358:c478935-478669 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTAGAGATTGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References