Detailed information of TA system TA074369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 515972..516619
• Replicon: chromosome
• Accession: NZ_CP020357
• Organism: Oceanobacillus iheyensis strain CHQ24

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0U9H5W3
• Locus tag: OBCHQ24_RS02720
• Protein ID: WP_036577165.1
• Coordinates: 516260..516619 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: OBCHQ24_RS02715
• Protein ID: WP_081779446.1
• Coordinates: 515972..516256 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OBCHQ24_RS02695	511135..511509	+	375	WP_106895723.1	holo-ACP synthase	-
OBCHQ24_RS02700	511554..513068	+	1515	WP_106895724.1	NAD(P)H-hydrate dehydratase	-
OBCHQ24_RS02705	513128..514144	+	1017	WP_106895725.1	outer membrane lipoprotein carrier protein LolA	-
OBCHQ24_RS02710	514682..515806	+	1125	WP_106895726.1	alanine racemase	-
OBCHQ24_RS02715	515972..516256	+	285	WP_081779446.1	antitoxin	Antitoxin
OBCHQ24_RS02720	516260..516619	+	360	WP_036577165.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OBCHQ24_RS02725	516905..517774	+	870	WP_106895727.1	RsbT co-antagonist protein RsbRA	-
OBCHQ24_RS02730	517779..518135	+	357	WP_036577167.1	STAS domain-containing protein	-
OBCHQ24_RS02735	518139..518540	+	402	WP_106895728.1	anti-sigma regulatory factor	-
OBCHQ24_RS02740	518664..519677	+	1014	WP_106895729.1	PP2C family protein-serine/threonine phosphatase	-
OBCHQ24_RS02745	519788..520120	+	333	WP_106895730.1	STAS domain-containing protein	-
OBCHQ24_RS02750	520120..520596	+	477	WP_106895731.1	anti-sigma B factor RsbW	-
OBCHQ24_RS02755	520568..521359	+	792	WP_106895732.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13368.57 Da
• Isoelectric Point: 9.4865

>T74369 WP_036577165.1 NZ_CP020357:516260-516619 [Oceanobacillus iheyensis]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDNEMMKKIDQALEISLGLKDMYGQ

Nucleotide

• Length: 360 bp

>T74369 NZ_CP020357:516260-516619 [Oceanobacillus iheyensis]
TTGATCGTTCAAAGAGGCGAAGTATACTTCGCTGACTTATCCCCTGTGGTTGGATCAGAGCAGGGAGGCGTCCGCCCAGT
ATTGATCCTACAAAATGATATTGGAAATCGGTTTAGTCCAACTGTAATTGTAGCTGCAATTACAGCACAAATTCAGAAAG
CCAAGTTGCCAACACACGTTGAGATCAACGCAAAGCGCTACGGATTTGACCGCAATTCTGTCATATTGCTAGAACAAATA
CGTACGCTAGATAAGCAGCGGCTTACGGATAAAATAACAAAGCTCGACAACGAAATGATGAAAAAAATTGATCAGGCATT
AGAAATTAGCCTCGGTTTAAAAGATATGTACGGCCAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10891.16 Da
• Isoelectric Point: 4.4075

>AT74369 WP_081779446.1 NZ_CP020357:515972-516256 [Oceanobacillus iheyensis]
MSESLQEVLVKIPKNLLNEVDGLMKHENSDLSDFICQATRNFLEHKRDEHIQQFHETMQRGYEEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT74369 NZ_CP020357:515972-516256 [Oceanobacillus iheyensis]
TTGTCAGAGAGTTTACAAGAAGTCTTGGTAAAGATACCTAAAAACCTGTTAAATGAGGTGGATGGATTAATGAAACATGA
AAATAGTGATTTGAGCGATTTCATCTGTCAGGCAACAAGAAACTTTTTAGAGCACAAGAGAGATGAACACATTCAGCAAT
TTCATGAAACAATGCAACGAGGCTATGAAGAAATGGGCCGCATTAATCTAACAATTGCTTCGGAAGCTTTTCAAGCTGAA
GAGGAGGCTGAAAACACCTTAGAGCGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0U9H5W3