Detailed information of TA system TA074360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1988755..1989284
• Replicon: chromosome
• Accession: NZ_CP020354
• Organism: Staphylococcus aureus strain FORC59

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: FORC59_RS10110
• Protein ID: WP_001103939.1
• Coordinates: 1988755..1989072 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IA75
• Locus tag: FORC59_RS10115
• Protein ID: WP_001058492.1
• Coordinates: 1989075..1989284 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC59_RS10090	1984760..1985401	-	642	WP_072466816.1	pathogenicity island protein	-
FORC59_RS10095	1985398..1985778	-	381	WP_000356927.1	hypothetical protein	-
FORC59_RS10100	1986108..1987808	-	1701	WP_033842989.1	DUF927 domain-containing protein	-
FORC59_RS10105	1987822..1988691	-	870	WP_033842991.1	primase alpha helix C-terminal domain-containing protein	-
FORC59_RS10110	1988755..1989072	-	318	WP_001103939.1	DUF1474 family protein	Toxin
FORC59_RS10115	1989075..1989284	-	210	WP_001058492.1	hypothetical protein	Antitoxin
FORC59_RS10120	1989277..1989423	-	147	WP_000708434.1	hypothetical protein	-
FORC59_RS10125	1989420..1989737	-	318	WP_000481967.1	helix-turn-helix domain-containing protein	-
FORC59_RS10130	1989742..1989960	-	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
FORC59_RS10135	1990133..1990807	+	675	WP_000620857.1	helix-turn-helix transcriptional regulator	-
FORC59_RS10140	1990821..1991993	+	1173	WP_000179346.1	site-specific integrase	-
FORC59_RS10145	1992062..1993678	-	1617	WP_000240642.1	chaperonin GroEL	-
FORC59_RS10150	1993754..1994038	-	285	WP_000917289.1	co-chaperone GroES	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1977902..1994038	16136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T74360 WP_001103939.1 NZ_CP020354:c1989072-1988755 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T74360 NZ_CP020354:c1989072-1988755 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGGATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8238.28 Da
• Isoelectric Point: 4.3718

>AT74360 WP_001058492.1 NZ_CP020354:c1989284-1989075 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT74360 NZ_CP020354:c1989284-1989075 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IA75