Detailed information of TA system TA074355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1437871..1438790
• Replicon: chromosome
• Accession: NZ_CP020352
• Organism: Bacillus paralicheniformis strain MDJK30

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BLMD_RS07310
• Protein ID: WP_020451047.1
• Coordinates: 1438044..1438790 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: BLMD_RS07305
• Protein ID: WP_020451046.1
• Coordinates: 1437871..1438044 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLMD_RS07285	1433290..1434387	+	1098	WP_020451042.1	mannonate dehydratase	-
BLMD_RS07290	1434363..1435211	+	849	WP_020451043.1	SDR family oxidoreductase	-
BLMD_RS07295	1435239..1436252	+	1014	WP_020451044.1	zinc-binding alcohol dehydrogenase family protein	-
BLMD_RS07300	1436304..1437575	+	1272	WP_020451045.1	MFS transporter	-
BLMD_RS07305	1437871..1438044	-	174	WP_020451046.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BLMD_RS07310	1438044..1438790	-	747	WP_020451047.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BLMD_RS07315	1438900..1439898	-	999	WP_020451048.1	inorganic phosphate transporter	-
BLMD_RS07320	1439911..1440528	-	618	WP_020451049.1	DUF47 domain-containing protein	-
BLMD_RS07325	1440860..1442617	+	1758	WP_020451051.1	gamma-glutamyltransferase	-
BLMD_RS07330	1442754..1443398	+	645	WP_020451052.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29435.89 Da
• Isoelectric Point: 4.6065

>T74355 WP_020451047.1 NZ_CP020352:c1438790-1438044 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T74355 NZ_CP020352:c1438790-1438044 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTTTTGGCGCTGGCTCTGTATGTAGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACGTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGGATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6926.00 Da
• Isoelectric Point: 10.9186

>AT74355 WP_020451046.1 NZ_CP020352:c1438044-1437871 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT74355 NZ_CP020352:c1438044-1437871 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA