Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4955385..4956973 Replicon chromosome
Accession NZ_CP020350
Organism Pectobacterium brasiliense strain SX309

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag B5S52_RS22230 Protein ID WP_080164242.1
Coordinates 4955654..4956973 (+) Length 440 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag B5S52_RS22225 Protein ID WP_103943261.1
Coordinates 4955385..4955654 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5S52_RS22200 4950908..4951459 + 552 WP_040038303.1 TetR/AcrR family transcriptional regulator -
B5S52_RS22205 4951490..4951900 - 411 WP_117590612.1 helix-turn-helix transcriptional regulator -
B5S52_RS22210 4952014..4952865 + 852 WP_080164238.1 SDR family oxidoreductase -
B5S52_RS22215 4952959..4953843 - 885 WP_080164239.1 LysR family transcriptional regulator -
B5S52_RS22220 4954056..4955192 + 1137 WP_080164241.1 alpha/beta hydrolase -
B5S52_RS22225 4955385..4955654 + 270 WP_103943261.1 helix-turn-helix domain-containing protein Antitoxin
B5S52_RS22230 4955654..4956973 + 1320 WP_080164242.1 type II toxin-antitoxin system HipA family toxin Toxin
B5S52_RS22235 4957238..4958737 + 1500 WP_080164244.1 ATP-binding protein -
B5S52_RS22240 4958734..4959372 + 639 WP_080164246.1 HNH endonuclease -
B5S52_RS22715 4959513..4959653 + 141 WP_164669121.1 hypothetical protein -
B5S52_RS22245 4959988..4960776 - 789 WP_039281386.1 hypothetical protein -
B5S52_RS22250 4961187..4961558 - 372 Protein_4296 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-104)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-66)


Sequences

Toxin        


Download         Length: 440 a.a.        Molecular weight: 49254.45 Da        Isoelectric Point: 6.6181

>T74349 WP_080164242.1 NZ_CP020350:4955654-4956973 [Pectobacterium brasiliense]
MAALDVYMNGYRVGILTKTGSGAHHFSYDANWLGLPGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
TDSTQPFDLLAKVGQDSVGALQLVPQGTPVHDIKKIEYKTLSEQQLENILTGYLSDAPLGMIDTEDDFRISIAGAQEKTA
LLYLDDRWCLPLNATPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSVDPERDRYRFMQAQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKMAMGLTGSRGKKYAIEQIFPRHFFQTAKVVGFARESMENILAEFAHTV
DTVITTVGSQLPVDFPTHISEAILAGLQARSRRLMSGWE

Download         Length: 1320 bp

>T74349 NZ_CP020350:4955654-4956973 [Pectobacterium brasiliense]
ATGGCTGCGCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGGGCACATCATTTTTC
CTATGATGCGAACTGGCTCGGATTGCCAGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAACTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAT
ACGGATTCCACCCAACCGTTTGATCTGCTGGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTGCAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATACAAAACCTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGGTATC
TGTCCGATGCCCCGCTGGGTATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCG
CTGCTGTATCTGGATGATCGCTGGTGCCTGCCTCTCAATGCCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGTTTGCTCATTGCCAAGGCAT
TTGGGTTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGCGTTGGCGGTGGAGCGTTTTGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTGATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGGCTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAATTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAAATGGCGATGGGATTAACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCCAAGGTCGTTGGGTTTGCCAGAGAATCGATGGAAAACATTCTGGCGGAGTTTGCCCACACCGTT
GATACCGTCATAACGACTGTTGGAAGTCAACTGCCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGATT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10062.40 Da        Isoelectric Point: 9.7281

>AT74349 WP_103943261.1 NZ_CP020350:4955385-4955654 [Pectobacterium brasiliense]
MMKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTTNNEAS
VASSWKEEW

Download         Length: 270 bp

>AT74349 NZ_CP020350:4955385-4955654 [Pectobacterium brasiliense]
ATGATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGGAA
AGTGGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGCTGGAAACCT
TCTTTAAAATCCTGTCGGCATTGAATTTAGAACTTACCGTTACGCCCAGGAATGTGGACACCACCAATAACGAAGCCTCT
GTTGCATCATCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References