Detailed information of TA system TA074345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 4412096..4412623
• Replicon: chromosome
• Accession: NZ_CP020350
• Organism: Pectobacterium brasiliense strain SX309

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B5S52_RS19745
• Protein ID: WP_080164107.1
• Coordinates: 4412096..4412371 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4V6ESH4
• Locus tag: B5S52_RS19750
• Protein ID: WP_039345626.1
• Coordinates: 4412375..4412623 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S52_RS19720	4407257..4408479	+	1223	Protein_3810	ABC transporter permease	-
B5S52_RS19725	4408495..4409998	+	1504	Protein_3811	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
B5S52_RS19730	4410021..4410725	+	705	WP_014913963.1	hypothetical protein	-
B5S52_RS19735	4410736..4411218	+	483	WP_014913962.1	hypothetical protein	-
B5S52_RS19740	4411536..4412048	-	513	WP_080164106.1	AAA family ATPase	-
B5S52_RS19745	4412096..4412371	-	276	WP_080164107.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B5S52_RS19750	4412375..4412623	-	249	WP_039345626.1	ribbon-helix-helix domain-containing protein	Antitoxin
B5S52_RS19755	4412806..4413923	+	1118	Protein_3817	IS3 family transposase	-
B5S52_RS19765	4414404..4415351	-	948	WP_080164109.1	hypothetical protein	-
B5S52_RS19770	4415509..4416749	-	1241	Protein_3819	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4406609..4431404	24795
	-	flank	IS/Tn	-	-	4413402..4413923	521

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10992.23 Da
• Isoelectric Point: 4.6972

>T74345 WP_080164107.1 NZ_CP020350:c4412371-4412096 [Pectobacterium brasiliense]
MEIRWTQKAQDDLECIYGFASQYSRQHADEVLDRLIIGTTGLADYPRIGVSQTRYEPREVRKILFDDYEVHYEIQHDTIY
IVDLWHTREDR

Nucleotide

• Length: 276 bp

>T74345 NZ_CP020350:c4412371-4412096 [Pectobacterium brasiliense]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTGGAATGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCAACA
TGCTGACGAGGTGTTAGATCGTTTGATCATCGGAACCACGGGGCTTGCTGACTACCCGAGAATCGGCGTATCTCAGACAA
GATATGAACCACGTGAGGTGAGAAAGATTTTGTTTGATGATTATGAAGTTCACTATGAAATTCAGCATGATACGATTTAT
ATCGTCGATCTGTGGCATACACGAGAAGACCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9021.41 Da
• Isoelectric Point: 8.7560

>AT74345 WP_039345626.1 NZ_CP020350:c4412623-4412375 [Pectobacterium brasiliense]
MGQPAKQTISAQIPAELAAAVESLAIELDRSKSWVIKEALTAMIEERERRHQRILKGLADVDAGRVVSHADVIDFANKLK
KS

Nucleotide

• Length: 249 bp

>AT74345 NZ_CP020350:c4412623-4412375 [Pectobacterium brasiliense]
ATGGGACAACCCGCGAAACAGACGATCAGTGCTCAGATACCCGCCGAACTTGCGGCTGCGGTTGAAAGCCTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAGCGGCGGCATCAGCGGA
TTTTGAAAGGTCTTGCTGATGTCGATGCGGGAAGAGTAGTAAGCCATGCAGATGTGATCGACTTCGCCAACAAACTGAAA
AAGTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A4V6ESH4