Detailed information of TA system TA074340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3851062..3851555
• Replicon: chromosome
• Accession: NZ_CP020350
• Organism: Pectobacterium brasiliense strain SX309

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: B5S52_RS17220
• Protein ID: WP_080163922.1
• Coordinates: 3851283..3851555 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0SK21
• Locus tag: B5S52_RS17215
• Protein ID: WP_012773205.1
• Coordinates: 3851062..3851280 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S52_RS17190	3847269..3848528	+	1260	WP_080163915.1	integrase arm-type DNA-binding domain-containing protein	-
B5S52_RS17195	3848635..3848829	-	195	WP_080163917.1	DUF3950 domain-containing protein	-
B5S52_RS17200	3849015..3849356	+	342	WP_039309297.1	hypothetical protein	-
B5S52_RS22485	3849948..3850118	-	171	WP_155121810.1	hypothetical protein	-
B5S52_RS17210	3850260..3851003	+	744	WP_080163920.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
B5S52_RS17215	3851062..3851280	+	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
B5S52_RS17220	3851283..3851555	+	273	WP_080163922.1	type II toxin-antitoxin system toxin CcdB	Toxin
B5S52_RS17225	3851619..3852769	+	1151	WP_155121799.1	IS3 family transposase	-
B5S52_RS17230	3853036..3853392	+	357	WP_080163924.1	hypothetical protein	-
B5S52_RS17235	3853521..3856178	+	2658	WP_080163925.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3847091..3856835	9744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-90)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10413.08 Da
• Isoelectric Point: 4.8134

>T74340 WP_080163922.1 NZ_CP020350:3851283-3851555 [Pectobacterium brasiliense]
MQFIVYLYKRASHYKMFVDVQSDIVDTPKRRMVIPLIESHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVI
TDLGDCADEI

Nucleotide

• Length: 273 bp

>T74340 NZ_CP020350:3851283-3851555 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCTATACAAACGTGCCAGCCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGACAC
ACCGAAGCGGCGCATGGTCATTCCGCTTATCGAATCACATCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCTCTGA
TCCGCATCGATGGCGAAGATTATCGACTGATGACGACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGATT
ACAGATCTTGGCGACTGTGCGGATGAGATCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8201.16 Da
• Isoelectric Point: 7.2038

>AT74340 WP_012773205.1 NZ_CP020350:3851062-3851280 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT74340 NZ_CP020350:3851062-3851280 [Pectobacterium brasiliense]
ATGAAACACCGCGTCAGCGTTACCGTAGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAAGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTCATTGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTAA

Structures

Antitoxin

• AlphaFold DB: E0SK21