Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3614318..3614943 Replicon chromosome
Accession NZ_CP020350
Organism Pectobacterium brasiliense strain SX309

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag B5S52_RS16185 Protein ID WP_005976087.1
Coordinates 3614740..3614943 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q6D809
Locus tag B5S52_RS16180 Protein ID WP_010282247.1
Coordinates 3614318..3614686 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5S52_RS16165 3609951..3613079 + 3129 WP_039511040.1 multidrug efflux RND transporter permease subunit -
B5S52_RS16170 3613310..3613558 + 249 WP_039274617.1 type B 50S ribosomal protein L31 -
B5S52_RS16175 3613576..3613719 + 144 WP_005976091.1 type B 50S ribosomal protein L36 -
B5S52_RS16180 3614318..3614686 + 369 WP_010282247.1 Hha toxicity modulator TomB Antitoxin
B5S52_RS16185 3614740..3614943 + 204 WP_005976087.1 hemolysin expression modulator Hha Toxin
B5S52_RS16200 3615809..3616129 + 321 WP_010282239.1 MGMT family protein -
B5S52_RS16205 3616149..3616730 - 582 WP_080163873.1 YbaY family lipoprotein -
B5S52_RS16210 3616943..3617806 + 864 WP_014914538.1 acyl-CoA thioesterase II -
B5S52_RS16215 3617943..3619229 - 1287 WP_014914537.1 ammonium transporter AmtB -
B5S52_RS16220 3619269..3619607 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T74338 WP_005976087.1 NZ_CP020350:3614740-3614943 [Pectobacterium brasiliense]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T74338 NZ_CP020350:3614740-3614943 [Pectobacterium brasiliense]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGCCTGGCAGAACTGACGATGAACAAAC
TCTACGACAAAGTCCCTACTGCAGTATGGCGATACGTTCGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14046.80 Da        Isoelectric Point: 4.4787

>AT74338 WP_010282247.1 NZ_CP020350:3614318-3614686 [Pectobacterium brasiliense]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENICTPAKT

Download         Length: 369 bp

>AT74338 NZ_CP020350:3614318-3614686 [Pectobacterium brasiliense]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
GACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGTATTAATGATGCTGAACTACGGCGTTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACATCTGTACGCCTGCTAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A086EI31

References