Detailed information of TA system TA074337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3110408..3110961
• Replicon: chromosome
• Accession: NZ_CP020350
• Organism: Pectobacterium brasiliense strain SX309

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: B5S52_RS14100
• Protein ID: WP_039508990.1
• Coordinates: 3110408..3110722 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6CEY1
• Locus tag: B5S52_RS14105
• Protein ID: WP_012769207.1
• Coordinates: 3110725..3110961 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S52_RS14070	3106445..3107920	+	1476	WP_039520633.1	sensor domain-containing diguanylate cyclase	-
B5S52_RS14075	3108003..3108170	-	168	WP_010280855.1	YqaE/Pmp3 family membrane protein	-
B5S52_RS14080	3108325..3108711	+	387	WP_039518958.1	lysozyme inhibitor LprI family protein	-
B5S52_RS14085	3108952..3109749	+	798	WP_039518956.1	DgsA anti-repressor MtfA	-
B5S52_RS14100	3110408..3110722	-	315	WP_039508990.1	CcdB family protein	Toxin
B5S52_RS14105	3110725..3110961	-	237	WP_012769207.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
B5S52_RS14110	3111152..3111493	-	342	Protein_2735	ArdC family protein	-
B5S52_RS14115	3112192..3114501	+	2310	WP_039558208.1	TerB N-terminal domain-containing protein	-
B5S52_RS14120	3114505..3115821	+	1317	WP_039508987.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11521.45 Da
• Isoelectric Point: 7.9859

>T74337 WP_039508990.1 NZ_CP020350:c3110722-3110408 [Pectobacterium brasiliense]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T74337 NZ_CP020350:c3110722-3110408 [Pectobacterium brasiliense]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGGCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCCAGTATCCCTGTCCAGGCCCTGGGC
GCGGTGTTTTGTGATGCTTCGCAGTATCGTTCTCAGGTAAAGGCTGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9013.03 Da
• Isoelectric Point: 6.4631

>AT74337 WP_012769207.1 NZ_CP020350:c3110961-3110725 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT74337 NZ_CP020350:c3110961-3110725 [Pectobacterium brasiliense]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACAATGACAGTAGAGCGAGCCTTACTGGTAAGAGCGCGTGAAGCGGG
TATTAACCTGAGTGCTACCCTGTCAACCGCACTGGATGCGGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTATCGACGCATTAAATCGTTTTCATGATGAAAACGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E4H0