Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 101275..101800 Replicon plasmid unnamed1
Accession NZ_CP020343
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag BS647_RS26130 Protein ID WP_001159860.1
Coordinates 101495..101800 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag BS647_RS26125 Protein ID WP_000813626.1
Coordinates 101275..101493 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS26080 (BS647_26195) 97001..97186 + 186 Protein_120 ATP-binding protein -
BS647_RS28040 (BS647_26200) 97286..97495 - 210 Protein_121 peptidoglycan-binding protein -
BS647_RS26090 (BS647_26205) 97545..97796 - 252 WP_015683204.1 transporter -
BS647_RS26100 (BS647_26215) 98248..98532 + 285 WP_011114774.1 ribbon-helix-helix domain-containing protein -
BS647_RS26105 (BS647_26220) 98532..98807 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
BS647_RS26120 (BS647_26235) 99786..100739 + 954 Protein_126 IS66 family transposase -
BS647_RS26125 (BS647_26240) 101275..101493 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BS647_RS26130 (BS647_26245) 101495..101800 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
BS647_RS26140 (BS647_26250) 102193..103830 + 1638 WP_000936806.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
BS647_RS26145 (BS647_26255) 104038..104307 + 270 Protein_130 type II toxin-antitoxin system antitoxin YacA -
BS647_RS28675 104307..104476 + 170 Protein_131 type II toxin-antitoxin system toxin YacB -
BS647_RS26150 (BS647_26260) 104559..105149 + 591 WP_000705601.1 type III secretion system effector protein kinase OspG -
BS647_RS26160 (BS647_26270) 105506..105772 + 267 Protein_133 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..144194 144194
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 86499..108623 22124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T74331 WP_001159860.1 NZ_CP020343:101495-101800 [Shigella flexneri 1a]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T74331 NZ_CP020343:101495-101800 [Shigella flexneri 1a]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT74331 WP_000813626.1 NZ_CP020343:101275-101493 [Shigella flexneri 1a]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT74331 NZ_CP020343:101275-101493 [Shigella flexneri 1a]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References