Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 98248..98807 Replicon plasmid unnamed1
Accession NZ_CP020343
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag BS647_RS26105 Protein ID WP_000421262.1
Coordinates 98532..98807 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A822PWA0
Locus tag BS647_RS26100 Protein ID WP_011114774.1
Coordinates 98248..98532 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS26065 (BS647_26180) 94173..94847 + 675 WP_004967157.1 IS66-like element accessory protein TnpA -
BS647_RS26070 (BS647_26185) 94844..95191 + 348 WP_000631708.1 IS66 family insertion sequence element accessory protein TnpB -
BS647_RS26075 (BS647_26190) 95211..96812 + 1602 WP_011110603.1 IS66-like element ISSfl3 family transposase -
BS647_RS26080 (BS647_26195) 97001..97186 + 186 Protein_120 ATP-binding protein -
BS647_RS28040 (BS647_26200) 97286..97495 - 210 Protein_121 peptidoglycan-binding protein -
BS647_RS26090 (BS647_26205) 97545..97796 - 252 WP_015683204.1 transporter -
BS647_RS26100 (BS647_26215) 98248..98532 + 285 WP_011114774.1 ribbon-helix-helix domain-containing protein Antitoxin
BS647_RS26105 (BS647_26220) 98532..98807 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BS647_RS26120 (BS647_26235) 99786..100739 + 954 Protein_126 IS66 family transposase -
BS647_RS26125 (BS647_26240) 101275..101493 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA -
BS647_RS26130 (BS647_26245) 101495..101800 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..144194 144194
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 86499..108623 22124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T74330 WP_000421262.1 NZ_CP020343:98532-98807 [Shigella flexneri 1a]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T74330 NZ_CP020343:98532-98807 [Shigella flexneri 1a]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10529.97 Da        Isoelectric Point: 6.2150

>AT74330 WP_011114774.1 NZ_CP020343:98248-98532 [Shigella flexneri 1a]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR

Download         Length: 285 bp

>AT74330 NZ_CP020343:98248-98532 [Shigella flexneri 1a]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB A0A822PWA0

References