Detailed information of TA system TA074329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 44795..45570
• Replicon: plasmid unnamed1
• Accession: NZ_CP020343
• Organism: Shigella flexneri 1a strain 0439

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: BS647_RS25745
• Protein ID: WP_000405245.1
• Coordinates: 44795..45277 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: BS647_RS25750
• Protein ID: WP_010921625.1
• Coordinates: 45268..45570 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BS647_RS25730 (BS647_25840)	41210..42901	+	1692	WP_000597733.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
BS647_RS25735 (BS647_25845)	42953..44161	+	1209	Protein_58	IS3 family transposase	-
BS647_RS25740 (BS647_25850)	44204..44727	-	524	Protein_59	IS3-like element ISSfl11 family transposase	-
BS647_RS25745 (BS647_25855)	44795..45277	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
BS647_RS25750 (BS647_25860)	45268..45570	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
BS647_RS25760 (BS647_25870)	45842..46114	+	273	WP_000019158.1	hypothetical protein	-
BS647_RS25765 (BS647_25875)	46095..47303	+	1209	WP_000198552.1	IS91 family transposase	-
BS647_RS28020	47475..47603	+	129	Protein_64	IS5/IS1182 family transposase	-
BS647_RS25775 (BS647_25885)	47752..49206	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
BS647_RS25780 (BS647_25890)	49610..50229	+	620	Protein_66	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA	1..144194	144194
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ospC2	32100..67201	35101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T74329 WP_000405245.1 NZ_CP020343:c45277-44795 [Shigella flexneri 1a]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T74329 NZ_CP020343:c45277-44795 [Shigella flexneri 1a]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT74329 WP_010921625.1 NZ_CP020343:c45570-45268 [Shigella flexneri 1a]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT74329 NZ_CP020343:c45570-45268 [Shigella flexneri 1a]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8