Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1985885..1986539 Replicon chromosome
Accession NZ_CP020342
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag BS647_RS10555 Protein ID WP_000244767.1
Coordinates 1986132..1986539 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag BS647_RS10550 Protein ID WP_000354052.1
Coordinates 1985885..1986151 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS10525 (1981055) 1981055..1981798 + 744 WP_000951957.1 SDR family oxidoreductase -
BS647_RS10530 (1981855) 1981855..1983288 - 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
BS647_RS10535 (1983333) 1983333..1983643 + 311 Protein_1897 N(4)-acetylcytidine aminohydrolase -
BS647_RS10540 (1983807) 1983807..1984466 + 660 WP_000250274.1 hemolysin III family protein -
BS647_RS10545 (1984662) 1984662..1985642 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BS647_RS10550 (1985885) 1985885..1986151 + 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
BS647_RS10555 (1986132) 1986132..1986539 + 408 WP_000244767.1 protein YgfX Toxin
BS647_RS10560 (1986579) 1986579..1987100 - 522 WP_001055867.1 flavodoxin FldB -
BS647_RS10565 (1987212) 1987212..1988108 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
BS647_RS10570 (1988133) 1988133..1988843 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BS647_RS10575 (1988849) 1988849..1990582 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T74312 WP_000244767.1 NZ_CP020342:1986132-1986539 [Shigella flexneri 1a]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T74312 NZ_CP020342:1986132-1986539 [Shigella flexneri 1a]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT74312 WP_000354052.1 NZ_CP020342:1985885-1986151 [Shigella flexneri 1a]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74312 NZ_CP020342:1985885-1986151 [Shigella flexneri 1a]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References