Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 14833..15355 Replicon plasmid unnamed2
Accession NZ_CP020341
Organism Shigella flexneri 4c strain 0702

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag BS653_RS25855 Protein ID WP_000220560.1
Coordinates 14833..15114 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag BS653_RS25860 Protein ID WP_000121743.1
Coordinates 15104..15355 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS653_RS25810 (BS653_25935) 10682..11848 - 1167 WP_000539529.1 MobP1 family relaxase -
BS653_RS25815 (BS653_25940) 11851..12396 - 546 WP_000757693.1 DNA distortion polypeptide 1 -
BS653_RS25820 (BS653_25945) 12722..12994 - 273 WP_000160399.1 hypothetical protein -
BS653_RS27185 (BS653_25950) 13007..13156 - 150 WP_000003880.1 hypothetical protein -
BS653_RS25830 (BS653_25955) 13226..13405 - 180 WP_000439078.1 hypothetical protein -
BS653_RS25835 (BS653_25960) 13447..13776 - 330 WP_000866648.1 hypothetical protein -
BS653_RS25840 (BS653_25965) 13869..14084 - 216 WP_052921379.1 hypothetical protein -
BS653_RS25845 (BS653_25970) 14074..14319 - 246 WP_001550454.1 hypothetical protein -
BS653_RS25850 (BS653_25975) 14364..14687 - 324 WP_001181903.1 hypothetical protein -
BS653_RS25855 (BS653_25980) 14833..15114 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BS653_RS25860 (BS653_25985) 15104..15355 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
BS653_RS25870 (BS653_25995) 16417..17271 + 855 WP_001239324.1 replication initiation protein -
BS653_RS25875 (BS653_26000) 17296..17742 + 447 WP_001074386.1 hypothetical protein -
BS653_RS25880 (BS653_26005) 17879..18235 + 357 WP_052921318.1 DNA distortion polypeptide 3 -
BS653_RS25885 (BS653_26010) 18232..19209 - 978 WP_024127745.1 hypothetical protein -
BS653_RS25890 (BS653_26015) 19234..19515 - 282 WP_001185482.1 hypothetical protein -
BS653_RS25895 (BS653_26020) 19613..20275 - 663 WP_000516402.1 division plane positioning ATPase MipZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / qnrS1 - 1..47688 47688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T74307 WP_000220560.1 NZ_CP020341:c15114-14833 [Shigella flexneri 4c]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T74307 NZ_CP020341:c15114-14833 [Shigella flexneri 4c]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT74307 WP_000121743.1 NZ_CP020341:c15355-15104 [Shigella flexneri 4c]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT74307 NZ_CP020341:c15355-15104 [Shigella flexneri 4c]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References