Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4364376..4364994 Replicon chromosome
Accession NZ_CP020339
Organism Shigella flexneri 4c strain 0702

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BS653_RS23610 Protein ID WP_001291435.1
Coordinates 4364376..4364594 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag BS653_RS23615 Protein ID WP_000344797.1
Coordinates 4364620..4364994 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS653_RS23580 (4360268) 4360268..4360579 - 312 WP_000409911.1 MGMT family protein -
BS653_RS23590 (4360958) 4360958..4361311 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BS653_RS23595 (4361353) 4361353..4362903 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BS653_RS23600 (4363067) 4363067..4363537 - 471 WP_000136192.1 YlaC family protein -
BS653_RS23605 (4363653) 4363653..4364204 - 552 Protein_4295 maltose O-acetyltransferase -
BS653_RS23610 (4364376) 4364376..4364594 - 219 WP_001291435.1 HHA domain-containing protein Toxin
BS653_RS23615 (4364620) 4364620..4364994 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
BS653_RS23620 (4365540) 4365540..4368689 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
BS653_RS23625 (4368712) 4368712..4369905 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74306 WP_001291435.1 NZ_CP020339:c4364594-4364376 [Shigella flexneri 4c]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74306 NZ_CP020339:c4364594-4364376 [Shigella flexneri 4c]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT74306 WP_000344797.1 NZ_CP020339:c4364994-4364620 [Shigella flexneri 4c]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74306 NZ_CP020339:c4364994-4364620 [Shigella flexneri 4c]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References